{"doi":"10.1016/j.jmb.2005.07.038","title":"Identification of a Universal VHH Framework to Graft Non-canonical Antigen-binding Loops of Camel Single-domain Antibodies","abstract":null,"journal":"Journal of Molecular Biology","year":2005,"id":617148,"datarank":9.241963905420356,"base_score":5.541263545158426,"endowment":5.541263545158426,"self_citation_contribution":0.831189531773764,"citation_network_contribution":8.410774373646591,"self_endowment_contribution":0.831189531773764,"citer_contribution":8.410774373646591,"corpus_percentile":null,"corpus_rank":null,"citation_count":254,"citer_count":200,"citers_with_citation_signal":195,"citers_with_endowment":195,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1591369,"name":"Mireille Pellis","orcid":null,"position":1,"is_corresponding":false},{"id":1591371,"name":"Remy Loris","orcid":null,"position":2,"is_corresponding":false},{"id":263120,"name":"Els Pardon","orcid":"0000-0002-2466-0172","position":3,"is_corresponding":false},{"id":1591374,"name":"Mireille Dumoulin","orcid":null,"position":4,"is_corresponding":false},{"id":1139088,"name":"André Matagne","orcid":"0000-0001-6417-6791","position":5,"is_corresponding":false},{"id":151398,"name":"Lode Wyns","orcid":null,"position":6,"is_corresponding":false},{"id":151396,"name":"Serge Muyldermans","orcid":null,"position":7,"is_corresponding":false},{"id":699107,"name":"Katja Conrath","orcid":"0000-0002-6759-0178","position":8,"is_corresponding":false},{"id":1591367,"name":"Dirk Saerens","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Identification of a Universal VHH Framework to Graft Non-canonical Antigen-binding Loops of Camel Single-domain Antibodies","abstract":"Camel single-domain antibody fragments (VHHs) are promising tools in numerous biotechnological and medical applications. However, some conditions under which antibodies are used are so demanding that they can be met by only the most robust VHHs. A universal framework offering the required properties for use in various applications (e.g. as intrabody, as probe in biosensors or on micro-arrays) is highly valuable and might be further implemented when employment of VHHs in human therapy is envisaged. We identified the VHH framework of cAbBCII10 as a potential candidate, useful for the exchange of antigen specificities by complementarity determining region (CDR) grafting. Due to the large number of CDR-H loop structures present on VHHs, this grafting technique was expected to be rather unpredictable. Nonetheless, the plasticity of the cAbBCII10 framework allows successful transfer of antigen specificity from donor VHHs onto its scaffold. The cAbBCII10 was chosen essentially for its high level of stability (47 kJmol(-1)), good expression level (5 mgl(-1) in E.coli) and its ability to be functional in the absence of the conserved disulfide bond. All five chimeras generated by grafting CDR-Hs, from donor VHHs belonging to subfamily 2 that encompass 75% of all antigen-specific VHHs, on the framework of cAbBCII10 were functional and generally had an increased thermodynamic stability. The grafting of CDR-H loops from VHHs belonging to other subfamilies resulted in chimeras of reduced antigen-binding capacity.","is_dataset_classified":null,"base_score":5.541263545158426,"endowment":5.541263545158426,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16095608","pmcid":null,"openalex_id":"https://openalex.org/W2016690055","authors":[],"funders":[],"total_grants":0,"fwci":3.0095,"citation_percentile":0.90346669,"influential_citations":0,"citation_trend":[{"year":2012,"count":8},{"year":2013,"count":6},{"year":2014,"count":14},{"year":2015,"count":12},{"year":2016,"count":9},{"year":2017,"count":15},{"year":2018,"count":15},{"year":2019,"count":10},{"year":2020,"count":15},{"year":2021,"count":10},{"year":2022,"count":24},{"year":2023,"count":15},{"year":2024,"count":23},{"year":2025,"count":28},{"year":2026,"count":9}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0022283605008260?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0022283605008260?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.jmb.2005.07.038","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16095608","host_type":"repository"},{"url":"https://orbi.uliege.be/handle/2268/22340","host_type":"repository"},{"url":"https://biblio.vub.ac.be/vubir/identification-of-a-universal-vhh-framework-to-graft-noncanonical-antigenbinding-loops-of-camel-singledomain-antibodies(908ba912-97b4-436e-8378-f2953b13f9fe).html","host_type":"repository"}],"fields_of_study":["Monoclonal and Polyclonal Antibodies Research","Protein purification and stability","Glycosylation and Glycoproteins Research"],"mesh_terms":["Amino Acid Sequence","Animals","Antigens","Binding Sites, Antibody","Biotechnology","Camelus","Immunoglobulin Variable Region","Immunoglobulin Heavy Chains","Models, Molecular","Molecular Sequence Data","Protein Conformation","Recombinant Fusion Proteins","Mutagenesis, Site-Directed","Sequence Homology, Amino Acid","Crystallography, X-Ray","Complementarity Determining Regions","In Vitro Techniques"],"keywords":["Single-domain antibody","Complementarity determining region","Computational biology","Antigen","Biology","Antibody","Chemistry","Biophysics","Molecular biology","Monoclonal antibody","Genetics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T00:57:15.780275Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}