{"doi":"10.1016/j.jlr.2024.100735","title":"Detecting white adipose tissue browning in mice with in vivo R2∗ mapping at 9.4T MRI","abstract":"White adipose tissue (WAT) browning is considered a promising strategy to combat obesity and related metabolic diseases. Currently, fat-water fraction (FWF) has been used as a marker for the loss of lipids associated with WAT browning. However, FWF may not be sensitive to metabolic changes and cannot specifically reflect iron-regulated metabolism during browning. Here, we report a noninvasive preclinical imaging approach based on iron content detected by R 2 ∗ mapping to assess in vivo WAT browning in mice. In this study, we investigated the browning of inguinal white adipose tissue (iWAT) induced by long-term CL-316,243 (CL) drug stimulation in mice. We quantified the changes in R 2 ∗, FWF, uncoupling protein 1 (UCP1) expression, and iron content. The iWAT of all mice was dissected for H&E staining and immunohistochemistry for the absorbance of UCP1 and iron content. In in vivo experiments, a significant increase in R 2 ∗ and a decrease in FWF were observed in iWAT after 7 days of CL administration compared with the saline-treated and the baseline groups. Accordingly, in ex vivo experiments, UCP1 expression and the total iron content in iWAT significantly increased after 7 days of CL stimulation. By pooling all mice data, the UCP1 expression level of iWAT and iron content was found to be highly correlated with R 2 ∗ and inversely correlated with FWF. Taken together, R 2 ∗ may serve as a potential imaging biomarker for assessing WAT browning, which provides a new diagnostic and therapeutic evaluation tool for metabolic diseases.","journal":"Journal of Lipid Research","year":2024,"id":472772,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9593,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1308252,"name":"Hongsheng Liu","orcid":"0000-0001-9242-6508","position":1,"is_corresponding":false},{"id":1215968,"name":"Yanqiu Feng","orcid":"0009-0000-5830-4697","position":2,"is_corresponding":false},{"id":1308682,"name":"Xiuwei Jiao","orcid":null,"position":3,"is_corresponding":false},{"id":1308253,"name":"Yuanbo Yang","orcid":"0009-0006-2098-2541","position":4,"is_corresponding":false},{"id":1308254,"name":"Daming Zhang","orcid":"0000-0002-5651-8699","position":5,"is_corresponding":false},{"id":1308255,"name":"Qian Wang","orcid":"0000-0003-0293-7369","position":6,"is_corresponding":false},{"id":1308683,"name":"Zoheb Ahasan","orcid":null,"position":7,"is_corresponding":false},{"id":337957,"name":"Andrew Li","orcid":"0000-0001-9933-7057","position":8,"is_corresponding":false},{"id":669231,"name":"Chong Wee Liew","orcid":"0000-0001-9904-0958","position":9,"is_corresponding":false},{"id":1308256,"name":"Zimeng Cai","orcid":"0000-0003-0583-5560","position":10,"is_corresponding":false},{"id":1215969,"name":"Zaiyi Liu","orcid":"0000-0002-9307-8522","position":11,"is_corresponding":false},{"id":416116,"name":"Kejia Cai","orcid":"0000-0003-1511-4273","position":12,"is_corresponding":false},{"id":1215967,"name":"Qiaoling Zhong","orcid":"0000-0002-1269-8106","position":0,"is_corresponding":true}],"reference_count":38,"raw_metadata":null,"created_at":"2026-07-19T02:05:57.032095Z","pmid":"39709160","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}