{"doi":"10.1016/j.jlr.2024.100520","title":"A fluorogenic substrate for the detection of lipid amidases in intact cells","abstract":"Lipid amidases of therapeutic relevance include acid ceramidase (AC), N-acylethanolamine-hydrolyzing acid amidase (NAAA) and fatty acid amide hydrolase (FAAH). Although fluorogenic substrates have been developed for the three enzymes and high throughput methods for screening have been reported, a platform for the specific detection of these enzyme activities in intact cells is lacking. In this article we report on the coumarinic 1-deoxydihydroceramide RBM1-151, a 1-deoxy derivative and vinilog of RBM14-C12, as a novel substrate of amidases. This compound is hydrolyzed by AC (appKm = 7.0 μM; appVmax = 99.3 nM/min), NAAA (appKm = 0.73 μM; appVmax = 0.24 nM/min), and FAAH (appKm = 3.6 μM; appVmax = 7.6 nM/min), but not by other ceramidases. We provide proof of concept that the use of RBM1-151 in combination with reported irreversible inhibitors of AC and FAAH allows the determination in parallel of the three amidase activities in single experiments in intact cells.","journal":"Journal of Lipid Research","year":2024,"id":483117,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9587,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":857822,"name":"Johnson Ung","orcid":"0000-0002-3275-0530","position":1,"is_corresponding":false},{"id":1324038,"name":"Alejandro Iñáñez","orcid":"0009-0007-0657-9278","position":2,"is_corresponding":false},{"id":1324377,"name":"Carine Dufau","orcid":null,"position":3,"is_corresponding":false},{"id":1324039,"name":"Kazuhito Tsuboi","orcid":"0000-0002-4943-944X","position":4,"is_corresponding":false},{"id":407082,"name":"Josefina Casas","orcid":"0000-0002-7926-5209","position":5,"is_corresponding":false},{"id":402541,"name":"Su‐Fern Tan","orcid":"0000-0002-8301-0823","position":6,"is_corresponding":false},{"id":366762,"name":"Thomas P. Loughran","orcid":"0000-0003-4981-1691","position":7,"is_corresponding":false},{"id":594905,"name":"Nathalie Andrieu‐Abadie","orcid":"0000-0003-2698-1970","position":8,"is_corresponding":false},{"id":1324378,"name":"Bruno Ségui","orcid":null,"position":9,"is_corresponding":false},{"id":366761,"name":"David J. Feith","orcid":"0000-0003-4363-8947","position":10,"is_corresponding":false},{"id":1128821,"name":"José Luı́s Abad","orcid":"0000-0002-8343-9611","position":11,"is_corresponding":false},{"id":467174,"name":"Gemma Fabriàs","orcid":"0000-0001-7162-3772","position":12,"is_corresponding":false},{"id":998587,"name":"Mireia Casasampere","orcid":"0000-0001-7757-1512","position":0,"is_corresponding":true}],"reference_count":55,"raw_metadata":null,"created_at":"2026-07-19T02:07:22.248464Z","pmid":"38369184","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}