{"doi":"10.1016/j.jim.2013.05.007","title":"Optimization and qualification of a memory B-cell ELISpot for the detection of vaccine-induced memory responses in HIV vaccine trials","abstract":null,"journal":"Journal of Immunological Methods","year":2013,"id":598631,"datarank":0.611630616585858,"base_score":4.07753744390572,"endowment":4.07753744390572,"self_citation_contribution":0.611630616585858,"citation_network_contribution":0.0,"self_endowment_contribution":0.611630616585858,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":58,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1534142,"name":"David P. Friedrich","orcid":null,"position":1,"is_corresponding":false},{"id":1193428,"name":"Julie A. Williams","orcid":"0000-0003-3467-0865","position":2,"is_corresponding":false},{"id":1534145,"name":"Rebecca J. Smith","orcid":null,"position":3,"is_corresponding":false},{"id":1084167,"name":"Terri L. Stewart","orcid":null,"position":4,"is_corresponding":false},{"id":1534147,"name":"Donald K. Carter","orcid":null,"position":5,"is_corresponding":false},{"id":433086,"name":"Hua-Xin Liao","orcid":null,"position":6,"is_corresponding":false},{"id":52019,"name":"M. Juliana McElrath","orcid":"0000-0003-2276-7117","position":7,"is_corresponding":false},{"id":355217,"name":"Nicole Frahm","orcid":"0000-0003-1298-3676","position":8,"is_corresponding":false},{"id":1534140,"name":"Paula N. Walsh","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Optimization and qualification of a memory B-cell ELISpot for the detection of vaccine-induced memory responses in HIV vaccine trials.","abstract":"Various aspects of the human immune system can be analyzed to determine the efficacy of a vaccine. We have developed a B-cell ELISpot to measure HIV-specific antibody-secreting B cells in the peripheral blood as a result of vaccination or natural infection. Our method includes stimulating peripheral blood mononuclear cells with interleukin-2 and a polyclonal activator, R848, to induce memory B cells to differentiate into antibody-secreting cells. Total immunoglobulin-secreting as well as antigen-specific B cells are then quantified. We have tested several HIV Env gp120 and gp140 proteins from different HIV subtypes, as well as a sensitive consensus group M Env gp140. Our findings indicate that the B-cell ELISpot provides a sensitive and specific tool to detect antigen-specific memory B-cell responses, and it is equally suited to detect antibody-secreting plasmablasts present in the circulation shortly after infection or vaccination.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23707324","pmcid":"PMC3736720","openalex_id":null,"authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"UM1 AI068618","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"U19 AI067854","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"UM1 AI068614","title":null}],"total_grants":3,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":null,"oa_locations":[],"fields_of_study":[],"mesh_terms":["B-Lymphocytes","Humans","AIDS Vaccines","Biotinylation","Antibody Formation","Immunologic Memory","Clinical Trials as Topic","Enzyme-Linked Immunospot Assay"],"keywords":["TMB","Immunoglobulin","Vaccine","PBMC","Antigen","HIV","Ig","Peripheral blood mononuclear cells","ASC","Ag","Klh","Pvdf","Hbss","Sfc","Polyvinylidene Fluoride","Antibody-secreting Cells","B-cell Elispot","Hanks Balanced Salt Solution","Keyhole Limpet Hemocyanin","Memory B-cell Responses","Mucosal Responses","Antibody-secreting Cell","Spot-forming Cells","Tetramethylbenzidine"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"gen"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T16:02:01.985490Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}