{"doi":"10.1016/j.jim.2006.07.015","title":"Statistical positivity criteria for the analysis of ELISpot assay data in HIV-1 vaccine trials","abstract":null,"journal":"Journal of Immunological Methods","year":2006,"id":595878,"datarank":0.637274286307404,"base_score":4.248495242049359,"endowment":4.248495242049359,"self_citation_contribution":0.637274286307404,"citation_network_contribution":0.0,"self_endowment_contribution":0.637274286307404,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":69,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":209603,"name":"Yunda Huang","orcid":null,"position":1,"is_corresponding":false},{"id":1105697,"name":"Lin Gu","orcid":"0000-0001-8435-0339","position":2,"is_corresponding":false},{"id":433168,"name":"John Hural","orcid":null,"position":3,"is_corresponding":false},{"id":608372,"name":"Steven G. Self","orcid":null,"position":4,"is_corresponding":false},{"id":307185,"name":"Zoe Moodie","orcid":"0000-0001-8701-2001","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Statistical positivity criteria for the analysis of ELISpot assay data in HIV-1 vaccine trials","abstract":"The enzyme-linked immunospot (ELISpot) assay is one of the leading technologies used to measure cellular responses in many settings including HIV vaccine clinical trials. HIV-1-specific effector T lymphocyte responses are considered necessary in the control of infection. Accurate measurement and summary of cellular immune responses are critical to HIV research. ELISpot assay readout is often dichotomized into positive/negative responses according to some pre-specified criteria. Given the increase in the number of replicates used for each stimulation with current assay configurations in the HIV Vaccine Trials Network (HVTN) laboratories, a new approach is now possible. We propose an objective criteria based on a hypothesis-driven method that controls the false positive rate while maintaining sensitivity to each of the antigen-specific responses under study. The new approach is compared to other commonly employed criteria using real and simulated data.","is_dataset_classified":null,"base_score":4.248495242049359,"endowment":4.248495242049359,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16959262","pmcid":null,"openalex_id":"https://openalex.org/W1980497671","authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"P01 AI57005","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI47086","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI48017","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"P30 AI27757","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI65328","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R37 AI35605","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"U01 AI46725","title":null}],"total_grants":7,"fwci":0.6687,"citation_percentile":0.61182033,"influential_citations":0,"citation_trend":[{"year":2012,"count":3},{"year":2013,"count":9},{"year":2014,"count":5},{"year":2015,"count":6},{"year":2016,"count":4},{"year":2017,"count":4},{"year":2018,"count":2},{"year":2020,"count":4},{"year":2021,"count":3},{"year":2022,"count":3},{"year":2023,"count":2},{"year":2024,"count":2},{"year":2025,"count":4},{"year":2026,"count":2}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0022175906002158?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0022175906002158?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.jim.2006.07.015","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16959262","host_type":"repository"}],"fields_of_study":["HIV Research and Treatment","Advanced Biosensing Techniques and Applications","Statistical Methods in Clinical Trials","AIDS Vaccines","Clinical Trials as Topic","Computer Simulation","Data Interpretation, Statistical","Enzyme-Linked Immunosorbent Assay","False Positive Reactions","HIV Seropositivity","HIV-1","Humans","Sensitivity and Specificity","T-Lymphocytes"],"mesh_terms":["Clinical Trials as Topic","Computer Simulation","Data Interpretation, Statistical","Enzyme-Linked Immunosorbent Assay","False Positive Reactions","HIV Seropositivity","Humans","Sensitivity and Specificity","T-Lymphocytes","HIV-1","AIDS Vaccines"],"keywords":["ELISPOT","HIV vaccine","Immunology","Medicine","Human immunodeficiency virus (HIV)","Clinical trial","Immune system","Antigen","Vaccine trial","T cell","Internal medicine"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-27T17:59:39.461403Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}