{"doi":"10.1016/j.jgar.2021.05.019","title":"Genomic analysis of carbapenem-resistant Pseudomonas aeruginosa ST143 clone showing susceptibility to broad-spectrum cephalosporins","abstract":"OBJECTIVES: Using whole-genome sequencing (WGS), we aimed to characterise a Pseudomonas aeruginosa ST143 clinical strain (Pb9) that presented resistance to meropenem and imipenem and susceptibility to piperacillin/tazobactam and broad-spectrum cephalosporins. METHODS: The antimicrobial susceptibility profile was confirmed by broth microdilution. WGS was performed using an Illumina MiSeq platform to identify possible genetic determinants of β-lactam resistance. Transcription levels of chromosomally encoded efflux systems and oprD were evaluated by RT-qPCR. RESULTS: were observed. In addition, we detected a premature stop codon in the major porin-encoding gene oprD coupled with hyperexpression of MexAB-OprM and MexEF-OprN. CONCLUSION: Our results suggest that the β-lactam resistance phenotype presented by strain Pb9 might be related to an association of OprD loss with hyperexpression of the efflux pump systems MexAB-OprM and MexEF-OprN. However, the contribution of OXA-488, PDC-5 and PIB-1 to this phenotype remains unclear and warrants further investigation.","journal":"Journal of Global Antimicrobial Resistance","year":2021,"id":185872,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.6845,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":743064,"name":"Rodrigo Cayô","orcid":"0000-0002-0709-6282","position":1,"is_corresponding":false},{"id":743065,"name":"Carolina Silva Nodari","orcid":"0000-0002-2361-5732","position":2,"is_corresponding":false},{"id":743066,"name":"Luiz Gonzaga Paula de Almeida","orcid":"0000-0002-7124-2715","position":3,"is_corresponding":false},{"id":518347,"name":"Fernanda Fernandes Santos","orcid":null,"position":4,"is_corresponding":false},{"id":232743,"name":"Blake Hanson","orcid":"0000-0003-2949-7818","position":5,"is_corresponding":false},{"id":232752,"name":"An Q Dinh","orcid":"0000-0002-2965-9269","position":6,"is_corresponding":false},{"id":699751,"name":"Ana Tereza Ribeiro de Vasconcelos","orcid":"0000-0002-4632-2086","position":7,"is_corresponding":false},{"id":743851,"name":"Willian R. Miller","orcid":null,"position":8,"is_corresponding":false},{"id":232742,"name":"César A. Arias","orcid":"0000-0001-9054-9774","position":9,"is_corresponding":false},{"id":657080,"name":"Ana Cristina Gales","orcid":"0000-0003-0913-768X","position":10,"is_corresponding":false},{"id":646892,"name":"Ana Paula Streling","orcid":"0000-0002-1662-0947","position":0,"is_corresponding":true}],"reference_count":13,"raw_metadata":null,"created_at":"2026-07-18T23:48:42.632147Z","pmid":"34175444","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}