{"doi":"10.1016/j.jff.2022.105363","title":"Synergistic effect of n-3 PUFA and probiotic supplementation on bone loss induced by chronic mild stress through the brain–gut–bone axis","abstract":"This study was to investigate the synergistic effect of n-3 polyunsaturated fatty acids (PUFA) and probiotics on depression-induced bone loss. Rats were fed with a diet composed of 0% or 1% n-3 PUFA and live or dead probiotics, and underwent chronic mild stress. N-3 PUFA with live or dead probiotics enhanced bone mass, calcium, and osteoprotegerin and decreased amino-terminal cross-linked telopeptide of type 1 collagen, receptor activator of nuclear factor kappa-Β ligand, and cytokines. N-3 PUFA and probiotics increased brain serotonin and modulated the hypothalamic–pituitary–adrenal axis, thereby decreasing the bone expression of beta 2 adrenergic receptor and activating transcription factor 4 and increasing runt-related factor 2. N-3 PUFA and probiotics decreased gut serotonin, which downregulated the bone expression of 5-hydroxytryptamine receptor 1B and increased cyclic adenosine monophosphate. This study showed the synergistic bone-protective effects of n-3 PUFA and probiotics through the brain–gut–bone axis.","journal":"Journal of Functional Foods","year":2022,"id":274792,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9544,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":943360,"name":"Haemin Oh","orcid":"0000-0002-1725-9276","position":1,"is_corresponding":false},{"id":943361,"name":"Miyea Jo","orcid":"0000-0003-0957-8952","position":2,"is_corresponding":false},{"id":943362,"name":"Hyunji Cho","orcid":"0000-0002-8476-2402","position":3,"is_corresponding":false},{"id":351750,"name":"Yongsoon Park","orcid":"0000-0001-5110-5716","position":4,"is_corresponding":false},{"id":943359,"name":"Yunjung Lee","orcid":"0000-0001-9878-3872","position":0,"is_corresponding":true}],"reference_count":57,"raw_metadata":null,"created_at":"2026-07-19T00:28:12.324621Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}