{"doi":"10.1016/j.jcyt.2023.01.008","title":"Label-free in vitro assays predict the potency of anti-disialoganglioside chimeric antigen receptor T-cell products","abstract":"BACKGROUND AIMS: Chimeric antigen receptor (CAR) T cells have demonstrated remarkable efficacy against hematological malignancies; however, they have not experienced the same success against solid tumors such as glioblastoma (GBM). There is a growing need for high-throughput functional screening platforms to measure CAR T-cell potency against solid tumor cells. METHODS: We used real-time, label-free cellular impedance sensing to evaluate the potency of anti-disialoganglioside (GD2) targeting CAR T-cell products against GD2+ patient-derived GBM stem cells over a period of 2 days and 7 days in vitro. We compared CAR T products using two different modes of gene transfer: retroviral transduction and virus-free CRISPR-editing. Endpoint flow cytometry, cytokine analysis and metabolomics data were acquired and integrated to create a predictive model of CAR T-cell potency. RESULTS: Results indicated faster cytolysis by virus-free CRISPR-edited CAR T cells compared with retrovirally transduced CAR T cells, accompanied by increased inflammatory cytokine release, CD8+ CAR T-cell presence in co-culture conditions and CAR T-cell infiltration into three-dimensional GBM spheroids. Computational modeling identified increased tumor necrosis factor α concentrations with decreased glutamine, lactate and formate as being most predictive of short-term (2 days) and long-term (7 days) CAR T cell potency against GBM stem cells. CONCLUSIONS: These studies establish impedance sensing as a high-throughput, label-free assay for preclinical potency testing of CAR T cells against solid tumors.","journal":"Cytotherapy","year":2023,"id":347482,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9584,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":506214,"name":"Maxwell B. Colonna","orcid":"0000-0002-0289-2546","position":1,"is_corresponding":false},{"id":253454,"name":"Katherine P. Mueller","orcid":"0000-0002-1873-3638","position":2,"is_corresponding":false},{"id":1089375,"name":"Divya Ventarapragada","orcid":null,"position":3,"is_corresponding":false},{"id":1088942,"name":"Riley Rodier","orcid":"0000-0002-7633-4096","position":4,"is_corresponding":false},{"id":716046,"name":"Chaitanya Tondepu","orcid":null,"position":5,"is_corresponding":false},{"id":256692,"name":"Nicole J. Piscopo","orcid":null,"position":6,"is_corresponding":false},{"id":815633,"name":"Amritava Das","orcid":null,"position":7,"is_corresponding":false},{"id":553140,"name":"Stacie Chvatal","orcid":null,"position":8,"is_corresponding":false},{"id":1089376,"name":"Heather B. Hayes","orcid":null,"position":9,"is_corresponding":false},{"id":277448,"name":"Christian M. Capitini","orcid":"0000-0002-2276-6731","position":10,"is_corresponding":false},{"id":69838,"name":"Daniel J. Brat","orcid":"0000-0002-0633-6563","position":11,"is_corresponding":false},{"id":1088943,"name":"Theresa Kotanchek","orcid":"0000-0002-0417-8864","position":12,"is_corresponding":false},{"id":282871,"name":"Arthur S. Edison","orcid":"0000-0002-5686-2350","position":13,"is_corresponding":false},{"id":253458,"name":"Krishanu Saha","orcid":"0000-0003-2837-0858","position":14,"is_corresponding":false},{"id":355143,"name":"Lohitash Karumbaiah","orcid":"0000-0001-7969-417X","position":15,"is_corresponding":false},{"id":355141,"name":"Meghan Logun","orcid":"0000-0002-7274-811X","position":0,"is_corresponding":true}],"reference_count":79,"raw_metadata":null,"created_at":"2026-07-19T01:11:57.720122Z","pmid":"36849306","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}