{"doi":"10.1016/j.jcmgh.2022.09.009","title":"PFKFB3-mediated Pro-glycolytic Shift in Hepatocellular Carcinoma Proliferation","abstract":"BACKGROUND & AIMS: Metabolic reprogramming, in particular, glycolytic regulation, supports abnormal survival and growth of hepatocellular carcinoma (HCC) and could serve as a therapeutic target. In this study, we sought to identify glycolytic regulators in HCC that could be inhibited to prevent tumor progression and could also be monitored in vivo, with the goal of providing a theragnostic alternative to existing therapies. METHODS: C MRI to metabolite levels from ex vivo mass spectrometry, mRNA levels of key glycolytic regulators, and histopathology. RESULTS: Glycolytic lactate flux increased within HCC tumors 3 days after tumor stimulation, correlating positively with tumor proliferation as measured with Ki67. This was associated with a shift towards aerobic glycolysis and downregulation of the pentose phosphate pathway detected by mass spectrometry. MRI-measured lactate flux was most closely coupled with PFKFB3 expression and was suppressed with direct inhibition using PFK15. CONCLUSIONS: C MRI.","journal":"Cellular and Molecular Gastroenterology and Hepatology","year":2022,"id":250758,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":26,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9649,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":718923,"name":"Aaron K. Grant","orcid":"0000-0002-2605-8062","position":1,"is_corresponding":false},{"id":262688,"name":"Cody Callahan","orcid":"0000-0002-0267-7096","position":2,"is_corresponding":false},{"id":719788,"name":"Patrícia Coutinho de Souza","orcid":null,"position":3,"is_corresponding":false},{"id":744130,"name":"David Mwin","orcid":null,"position":4,"is_corresponding":false},{"id":385106,"name":"Adam L. Booth","orcid":"0000-0002-1231-4117","position":5,"is_corresponding":false},{"id":315165,"name":"Imad Nasser","orcid":"0000-0003-1228-2474","position":6,"is_corresponding":false},{"id":461529,"name":"Marwan Moussa","orcid":"0000-0003-3462-7007","position":7,"is_corresponding":false},{"id":743403,"name":"Muneeb Ahmed","orcid":"0000-0003-0323-8260","position":8,"is_corresponding":false},{"id":381213,"name":"Leo L. Tsai","orcid":"0000-0001-6941-7443","position":9,"is_corresponding":false},{"id":471985,"name":"Qianhui Dou","orcid":"0000-0002-2418-4858","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":null,"created_at":"2026-07-19T00:24:32.960657Z","pmid":"36162723","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}