{"doi":"10.1016/j.jcf.2013.11.002","title":"Reassessment of the importance of mucins in determining sputum properties in cystic fibrosis","abstract":null,"journal":"Journal of Cystic Fibrosis","year":2014,"id":593807,"datarank":0.4636563680037475,"base_score":3.091042453358316,"endowment":3.091042453358316,"self_citation_contribution":0.4636563680037475,"citation_network_contribution":0.0,"self_endowment_contribution":0.4636563680037475,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":21,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1519855,"name":"Karine Rousseau","orcid":null,"position":1,"is_corresponding":false},{"id":1519856,"name":"Caroline Ridley","orcid":null,"position":2,"is_corresponding":false},{"id":1519857,"name":"William Flight","orcid":null,"position":3,"is_corresponding":false},{"id":497754,"name":"Andrew Jones","orcid":"0000-0001-7340-6499","position":4,"is_corresponding":false},{"id":1519858,"name":"Thomas A. Waigh","orcid":null,"position":5,"is_corresponding":false},{"id":612927,"name":"David J. Thornton","orcid":"0000-0001-7148-1970","position":6,"is_corresponding":false},{"id":722207,"name":"Alex Horsley","orcid":"0000-0003-1828-0058","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Reassessment of the importance of mucins in determining sputum properties in cystic fibrosis","abstract":"BACKGROUND: There is conflicting evidence about the importance of airway mucins (MUC5AC and MUC5B) in determining physical properties of sputum in cystic fibrosis (CF). We studied the effects of endogenous degradation of mucins on CF sputum elasticity and apparent mucin concentrations. METHODS: Elastic shear moduli (G') and mucin concentrations in sputum of 12 CF patients were measured before and after incubation at 37°C for 60min. RESULTS: G' fell from a median of 5.98 to 4.70Pa (p=0.01). There were significant falls in MUC5AC (8.2 to 5.2μg/ml, p=0.02) and MUC5B (17.3 to 12.5μg/ml, p=0.02) over the same period, and associated decrease in molecular weight and size. CONCLUSIONS: Sputum is not inert and degradation reduces apparent mucin concentrations and sputum elasticity. Even if care is taken to process samples rapidly, sputum may therefore differ from secretions retained in airways. Previous studies may have underestimated the role of mucins in CF sputum.","is_dataset_classified":null,"base_score":3.091042453358316,"endowment":3.091042453358316,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24332705","pmcid":"PMC3994278","openalex_id":"https://openalex.org/W2133687353","authors":[],"funders":[{"funder_name":"National Institute for Health Research (NIHR)","grant_id":"NIHR-CS-012-013","title":null},{"funder_name":"Wellcome Trust","grant_id":"088785/Z/09/Z","title":null},{"funder_name":"Medical Research Council","grant_id":"G1000450","title":null},{"funder_name":"Wellcome Trust","grant_id":"088785","title":"Wellcome Trust Centre for Cell-Matrix Research core award renewal."},{"funder_name":"Wellcome Trust","grant_id":"","title":null},{"funder_name":"Wellcome Trust","grant_id":"","title":null}],"total_grants":6,"fwci":0.8729,"citation_percentile":0.77192454,"influential_citations":0,"citation_trend":[{"year":2014,"count":1},{"year":2016,"count":3},{"year":2017,"count":4},{"year":2018,"count":4},{"year":2019,"count":3},{"year":2022,"count":3},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"http://www.cysticfibrosisjournal.com/article/S1569199313001902/pdf","host_type":"journal"},{"url":"http://www.cysticfibrosisjournal.com/article/S1569199313001902/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1569199313001902?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1569199313001902?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.jcf.2013.11.002","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24332705","host_type":"repository"},{"url":"https://research.manchester.ac.uk/en/publications/30f7e96d-70b9-4f31-be96-8d6a61a4600a","host_type":"repository"},{"url":"http://europepmc.org/articles/PMC3994278","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3994278","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC3994278","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1016/j.jcf.2013.11.002","host_type":""},{"url":"https://dx.doi.org/10.1016/j.jcf.2013.11.002","host_type":""},{"url":"http://www.elsevier.com/locate/jcf","host_type":""}],"fields_of_study":["Cystic Fibrosis Research Advances","Inhalation and Respiratory Drug Delivery","Asthma and respiratory diseases","0301 basic medicine","03 medical and health sciences","0303 health sciences","Adolescent","Adult","Cystic Fibrosis","Elasticity","Female","Humans","Male","Molecular Weight","Mucin 5AC","Mucin-5B","Pneumonia","Proteolysis","Rheology","Spirometry","Sputum","Young Adult"],"mesh_terms":["Adolescent","Adult","Cystic Fibrosis","Elasticity","Female","Humans","Male","Molecular Weight","Pneumonia","Rheology","Spirometry","Sputum","Mucin-5B","Mucin 5AC","Young Adult","Proteolysis"],"keywords":["Mucin","Sputum","Cystic fibrosis","Medicine","Airway","Gastroenterology","Microbiology","Internal medicine","Pathology","Surgery","Biology","Proteolysis","Lung Inflammation","Pulmonary and Respiratory Medicine","Adult","Male","Adolescent","Mucin 5AC","Young Adult","Humans","Pediatrics, Perinatology, and Child Health","Pneumonia","Mucin-5B","Elasticity","Molecular Weight","Spirometry","Original Article","Female","Rheology"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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