{"doi":"10.1016/j.jbiosc.2015.01.019","title":"Sequence modifications and heterologous expression of eukaryotic cytochromes P450 in Escherichia coli","abstract":null,"journal":"Journal of Bioscience and Bioengineering","year":2015,"id":652747,"datarank":0.4887144807032224,"base_score":3.258096538021482,"endowment":3.258096538021482,"self_citation_contribution":0.4887144807032224,"citation_network_contribution":0.0,"self_endowment_contribution":0.4887144807032224,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":25,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1702905,"name":"Mayumi Hatakeyama","orcid":null,"position":1,"is_corresponding":false},{"id":1702906,"name":"Yukiyo Yamauchi","orcid":null,"position":2,"is_corresponding":false},{"id":1702904,"name":"Hirofumi Ichinose","orcid":"0000-0001-6429-7323","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Sequence modifications and heterologous expression of eukaryotic cytochromes P450 in Escherichia coli","abstract":"The development of a heterologous expression system is often a principal step in biochemical and biotechnological studies on cytochromes P450 (P450s). However, heterologous expression of eukaryotic membrane-bound P450s in Escherichia coli is still a trial-and-error process because theoretical and systematical experimental procedures have not yet been established. In this study, we generated a series of chimeric variants of fungal P450s by replacing their N-terminal domains with the N-terminal domains of other P450s and explored their potentials for heterologous expression in E. coli. Large-scale screening demonstrated that a number of fungal P450s could be expressed when their N-terminal amino acid sequences were replaced with the corresponding domain of CYP5144C1, even when the expression of the non-chimeric sequence was unpromising. Furthermore, a comprehensive screening resulted in the identification of 64 different types of chimeric partners whose N-terminal domains could potentially be used to increase the expression levels of various P450s. These findings will help to elaborate experimental strategies for high-level heterologous expression of a variety of eukaryotic membrane-bound P450s in E. coli.","is_dataset_classified":null,"base_score":3.258096538021482,"endowment":3.258096538021482,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"25732206","pmcid":null,"openalex_id":"https://openalex.org/W2131381401","authors":[],"funders":[{"funder_name":"Ministry of Education, Culture, Sports, Science and Technology","grant_id":"24688034","title":null}],"total_grants":1,"fwci":1.9631,"citation_percentile":0.85654517,"influential_citations":0,"citation_trend":[{"year":2016,"count":3},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2020,"count":1},{"year":2021,"count":5},{"year":2022,"count":2},{"year":2023,"count":5},{"year":2024,"count":4},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S1389172315000584?httpAccept=text/plain","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1389172315000584?httpAccept=text/xml","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.jbiosc.2015.01.019","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/25732206","host_type":"repository"}],"fields_of_study":["Pharmacogenetics and Drug Metabolism","Microbial Metabolic Engineering and Bioproduction","Microbial Natural Products and Biosynthesis"],"mesh_terms":["Cytochrome P-450 Enzyme System","Escherichia coli","Fungi","Protein Structure, Tertiary","Mutant Chimeric Proteins"],"keywords":["Heterologous","Escherichia coli","Heterologous expression","Biology","Computational biology","Fusion protein","Biochemistry","Cell biology","Recombinant DNA","Gene","N-terminal domain","Chimerization","Eukaryotic Cytochrome P450","Sequence Modification"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T16:50:11.131791Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}