{"doi":"10.1016/j.jbc.2025.110834","title":"Endophilin–lamellipodin–VASP, key components in fast endophilin–mediated endocytosis, control actin polymerization within liquid-like condensates","abstract":"Actin polymerization is essential in several clathrin-independent endocytic pathways including fast endophilin-mediated endocytosis (FEME); however, the actin machinery involved in FEME has been elusive. Here, we show that the actin polymerase VASP colocalizes and interacts directly with the FEME priming complex. We identify endophilin as a VASP binding partner and establish novel noncanonical interactions between the SH3 domain of endophilin and the Ena/VASP homology 1 or 2 (EVH1 and EVH2) domains of VASP. We show that liquid-like condensates formed by endophilin, lamellipodin, and VASP localize actin polymerization both in solution and on lipid membranes and result in the formation of torus-shaped condensates in the bulk. We establish a new role for multivalent endophilin-lamellipodin interactions in the regulation of actin polymerization, and we identify a novel function for endophilin as a promoter of actin bundling. Our findings support a model that explains the connection between local actin polymerization and dynamic formation and dissolution of endocytic priming patches in FEME.","journal":"Journal of Biological Chemistry","year":2025,"id":554376,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9549,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":809695,"name":"Honey Priya James","orcid":null,"position":1,"is_corresponding":false},{"id":1452184,"name":"Jonathan F. W. Cope","orcid":null,"position":2,"is_corresponding":false},{"id":384988,"name":"Samsuzzoha Mondal","orcid":"0000-0002-2612-5974","position":3,"is_corresponding":false},{"id":1452185,"name":"Laura Baeyens","orcid":null,"position":4,"is_corresponding":false},{"id":1188386,"name":"Francesco Milano","orcid":"0000-0001-5453-2051","position":5,"is_corresponding":false},{"id":809179,"name":"Jason Zheng","orcid":"0000-0001-6652-7022","position":6,"is_corresponding":false},{"id":1064842,"name":"Matthias Krause","orcid":"0000-0002-3200-3199","position":7,"is_corresponding":false},{"id":384992,"name":"Tobias Baumgart","orcid":"0000-0001-7385-8460","position":8,"is_corresponding":false},{"id":384989,"name":"Karthik Narayan","orcid":"0000-0003-1047-0375","position":0,"is_corresponding":true}],"reference_count":108,"raw_metadata":null,"created_at":"2026-07-19T02:54:50.112989Z","pmid":"41109347","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}