{"doi":"10.1016/j.jbc.2024.107996","title":"The molecular chaperone ALYREF promotes R-loop resolution and maintains genome stability","abstract":"Unscheduled R-loops usually cause DNA damage and replication stress, and are therefore a major threat to genome stability. Several RNA processing factors, including the conserved THO complex and its associated RNA and DNA-RNA helicase UAP56, prevent R-loop accumulation in cells. Here, we investigate the function of ALYREF, an RNA export adapter associated with UAP56 and the THO complex, in R-loop regulation. We demonstrate that purified ALYREF promotes UAP56-mediated R-loop dissociation in vitro, and this stimulation is dependent on its interaction with UAP56 and R-loops. Importantly, we show that ALYREF binds DNA-RNA hybrids and R-loops. Consistently, ALYREF depletion causes R-loop accumulation and R-loop-mediated genome instability in cells. We propose that ALYREF, apart from its known role in RNA metabolism and export, is a key cellular R-loop coregulator, which binds R-loops and stimulates UAP56-driven resolution of unscheduled R-loops during transcription.","journal":"Journal of Biological Chemistry","year":2024,"id":437442,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9571,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1056153,"name":"Cristina Guillén-Mendoza","orcid":"0000-0003-4396-4865","position":1,"is_corresponding":false},{"id":1246917,"name":"Kathryn Primrose Banks","orcid":null,"position":2,"is_corresponding":false},{"id":1246918,"name":"Lillian Eliaz","orcid":null,"position":3,"is_corresponding":false},{"id":1246919,"name":"Sierra Southwell","orcid":null,"position":4,"is_corresponding":false},{"id":1246920,"name":"Darriel Eyaa","orcid":null,"position":5,"is_corresponding":false},{"id":1056155,"name":"Rosa Luna","orcid":"0000-0002-6131-6610","position":6,"is_corresponding":false},{"id":550247,"name":"Andrés Aguilera","orcid":"0000-0003-4782-1714","position":7,"is_corresponding":false},{"id":380566,"name":"Xiaoyu Xue","orcid":"0000-0001-9654-6566","position":8,"is_corresponding":false},{"id":1056151,"name":"Jay Bhandari","orcid":"0000-0002-3054-0011","position":0,"is_corresponding":true}],"reference_count":81,"raw_metadata":null,"created_at":"2026-07-19T02:00:29.872940Z","pmid":"39547511","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}