{"doi":"10.1016/j.jbc.2024.107410","title":"A real-time biochemical assay for quantitative analyses of APOBEC-catalyzed DNA deamination","abstract":"Over the past decade, the connection between APOBEC3 cytosine deaminases and cancer mutagenesis has become increasingly apparent. This growing awareness has created a need for biochemical tools that can be used to identify and characterize potential inhibitors of this enzyme family. In response to this challenge, we have developed a Real-time APOBEC3-mediated DNA Deamination assay. This assay offers a single-step set-up and real-time fluorescent read-out, and it is capable of providing insights into enzyme kinetics. The assay also offers a high-sensitivity and easily scalable method for identifying APOBEC3 inhibitors. This assay serves as a crucial addition to the existing APOBEC3 biochemical and cellular toolkit and possesses the versatility to be readily adapted into a high-throughput format for inhibitor discovery.","journal":"Journal of Biological Chemistry","year":2024,"id":463281,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9571,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":257750,"name":"Michael A. Carpenter","orcid":"0000-0001-9524-5323","position":1,"is_corresponding":false},{"id":401864,"name":"Yanjun Chen","orcid":"0000-0001-9030-5673","position":2,"is_corresponding":false},{"id":554546,"name":"William L. Brown","orcid":"0000-0001-5354-9468","position":3,"is_corresponding":false},{"id":464337,"name":"Nicholas H. Moeller","orcid":null,"position":4,"is_corresponding":false},{"id":1293771,"name":"Ian T Boylan","orcid":null,"position":5,"is_corresponding":false},{"id":1293772,"name":"Reuben S Harris","orcid":null,"position":6,"is_corresponding":false},{"id":103759,"name":"Hideki Aihara","orcid":"0000-0001-7508-6230","position":7,"is_corresponding":false},{"id":805260,"name":"Christopher Belica","orcid":"0000-0001-8492-4427","position":0,"is_corresponding":true}],"reference_count":30,"raw_metadata":null,"created_at":"2026-07-19T02:04:28.838123Z","pmid":"38796062","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}