{"doi":"10.1016/j.jbc.2022.102687","title":"Export of polybasic motif–containing secretory proteins BMP8A and SFRP1 from the endoplasmic reticulum is regulated by surfeit locus protein 4","abstract":null,"journal":"Journal of Biological Chemistry","year":2022,"id":590113,"datarank":0.49357550720945886,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"self_citation_contribution":0.3596842909197557,"citation_network_contribution":0.13389121628970319,"self_endowment_contribution":0.3596842909197557,"citer_contribution":0.13389121628970319,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":8,"citers_with_citation_signal":6,"citers_with_endowment":6,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1423471,"name":"Tingxuan Wang","orcid":"0000-0002-9985-3569","position":1,"is_corresponding":false},{"id":1085592,"name":"Yusong Guo","orcid":"0000-0002-5539-599X","position":2,"is_corresponding":false},{"id":1468704,"name":"Xiao Tang","orcid":"0000-0002-6601-4742","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Export of polybasic motif–containing secretory proteins BMP8A and SFRP1 from the endoplasmic reticulum is regulated by surfeit locus protein 4","abstract":"In the conventional secretory pathway, cargo receptors play important roles in exporting newly synthesized secretory proteins from the endoplasmic reticulum (ER). We previously showed that a cargo receptor, surfeit locus protein 4 (SURF4), promotes ER export of a soluble signaling molecule, sonic hedgehog, via recognizing the polybasic residues within its Cardin-Weintraub motif. In addition to sonic hedgehog, we found 30 more secretory proteins containing the polybasic motif (K/R)(K/R)(K/R)XX(K/R)(K/R), but whether SURF4 plays a general role in mediating ER export of these secretory proteins is unclear. Here, we analyzed the trafficking of four of these secretory proteins: desert hedgehog, Indian hedgehog, bone morphogenetic protein 8A (BMP8A), and secreted frizzled-related protein 1 (SFRP1). We found that the polybasic motifs contained in these cargo proteins are important for their ER export. Further analyses indicated that the polybasic motifs of BMP8A and SFRP1 interact with the triacidic motif on the predicted first luminal domain of SURF4. These interactions with SURF4 are essential and sufficient for the ER-to-Golgi trafficking of BMP8A and SFRP1. Moreover, we demonstrated that SURF4 localizes at a subpopulation of ER exit sites to regulate the ER export of its clients. Taken together, these results suggest that SURF4 is recruited to specific ER exit sites and plays a general role in capturing polybasic motif-containing secretory cargo proteins through electrostatic interactions.","is_dataset_classified":null,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"36370847","pmcid":"PMC9731852","openalex_id":"https://openalex.org/W4308616326","authors":[],"funders":[{"funder_name":"Southern Marine Science and Engineering Guangdong Laboratory","grant_id":"SMSEGL20SC01","title":null},{"funder_name":"Research Grants Council, University Grants Committee","grant_id":"16102921","title":null},{"funder_name":"Research Grants Council, University Grants Committee","grant_id":"16103319","title":null},{"funder_name":"Research Grants Council, University Grants Committee","grant_id":"16104020","title":null},{"funder_name":"Research Grants Council, University Grants Committee","grant_id":"C4002-20W","title":null},{"funder_name":"Research Grants Council, University Grants Committee","grant_id":"T13-602/21-N","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"NSFC31871421","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"NSFC32070699","title":null},{"funder_name":"Hong Kong University of Science and Technology","grant_id":"","title":null}],"total_grants":9,"fwci":0.8601,"citation_percentile":0.67792708,"influential_citations":0,"citation_trend":[{"year":2023,"count":4},{"year":2024,"count":3},{"year":2025,"count":1},{"year":2026,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.1016/j.jbc.2022.102687","host_type":"journal"},{"url":"https://doi.org/10.1016/j.jbc.2022.102687","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925822011309?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0021925822011309?httpAccept=text/plain","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/36370847","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9731852","host_type":"repository"},{"url":"http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=LinksAMR&SrcApp=PARTNER_APP&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000896945900008","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC9731852","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC9731852?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["Cellular transport and secretion","Genetic and Kidney Cyst Diseases","Pancreatic function and diabetes","Humans","Bone Morphogenetic Proteins","Endoplasmic Reticulum","Golgi Apparatus","Hedgehog Proteins","Intercellular Signaling Peptides and Proteins","Membrane Proteins","Protein Transport","Amino Acid Motifs","Secreted Frizzled-Related Proteins"],"mesh_terms":["Secreted Frizzled-Related Proteins","Endoplasmic Reticulum","Golgi Apparatus","Humans","Membrane Proteins","Bone Morphogenetic Proteins","Amino Acid Motifs","Protein Transport","Intercellular Signaling Peptides and Proteins","Hedgehog Proteins"],"keywords":["Endoplasmic reticulum","Secretory pathway","Secretory protein","Cell biology","Motif (music)","Locus (genetics)","Chemistry","Biology","Gene","Biochemistry","Golgi apparatus","Secretion","Cargo Sorting","Copii","Er Export","Surf4"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-24T13:19:52.222999Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}