{"doi":"10.1016/j.jbc.2022.102053","title":"A novel Cbx1, PurB, and Sp3 complex mediates long-term silencing of tissue- and lineage-specific genes","abstract":"miRNA-based cellular fate reprogramming offers an opportunity to investigate the mechanisms of long-term gene silencing. To further understand how genes are silenced in a tissue-specific manner, we leveraged our miRNA-based method of reprogramming fibroblasts into cardiomyocytes. Through screening approaches, we identified three proteins that were downregulated during reprogramming of fibroblasts into cardiomyocytes: heterochromatin protein Cbx1, transcriptional activator protein PurB, that of Cbx1, PurB, to gene in gene to Cbx1, PurB, fibroblasts to into in that Cbx1, PurB, a were to to genes the we that the of genes to an the we that Cbx1, PurB, fate miRNA-based cellular fate reprogramming offers an opportunity to investigate the mechanisms of long-term gene silencing. To further understand how genes are silenced in a tissue-specific manner, we leveraged our miRNA-based method of reprogramming fibroblasts into cardiomyocytes. Through screening approaches, we identified three proteins that were downregulated during reprogramming of fibroblasts into cardiomyocytes: heterochromatin protein Cbx1, transcriptional activator protein PurB, that of Cbx1, PurB, to gene in gene to Cbx1, PurB, fibroblasts to into in that Cbx1, PurB, a were to to genes the we that the of genes to an the we that Cbx1, PurB, fate reprogramming the to cellular reprogramming to of reprogramming a to in to miRNA-based we were to that fibroblasts into cardiomyocytes. Through a screening we that fibroblasts into a of we in in reprogramming of fibroblasts to fibroblasts into the an in in reprogramming of fibroblasts to our we to that in reprogramming in we that reprogramming reprogramming to reprogramming fibroblasts to to to reprogramming to to reprogramming of fibroblasts to approaches, miRNA-based reprogramming the of a of reprogramming in of the of reprogramming of miRNA-based reprogramming that mechanisms reprogramming in of the of reprogramming the of genes in are that are during are silenced tissue-specific genes are an of in in of gene to long-term gene of the of long-term that long-term gene of of the of long-term the the of mechanisms of gene of during mechanisms of in how are to the that to are the mechanisms miRNA-based reprogramming to into how genes are that a of fibroblasts into in in reprogramming of fibroblasts to reprogramming in reprogramming of the of reprogramming the reprogramming of fibroblasts into of reprogramming in reprogramming to our miRNA-based reprogramming method to understand how tissue-specific genes are an screening we identified three Cbx1, PurB, to Cbx1, PurB, to fibroblasts into in that Cbx1, PurB, a to to of an the of the that a of we fibroblasts into in in reprogramming of fibroblasts to reprogramming in that the of that of genes the of To we our that in the of in Through we that were proteins in the to were Cbx1, PurB, Cbx1, PurB, were to of the of in the of were of of the in of genes were the of of the of gene the of Cbx1, PurB, the of of the genes in Cbx1, PurB, to of the were of Cbx1, PurB, to fibroblasts into of the protein to of Cbx1, PurB, gene in fibroblasts further investigate the of Cbx1, PurB, we the of gene of Cbx1, PurB, gene of Cbx1, PurB, the of genes that the fibroblasts were the of Cbx1, PurB, gene were in fibroblasts the of a of Cbx1, PurB, gene of Cbx1, PurB, fibroblasts were a a of Cbx1, PurB, of the genes a of genes were were to the cardiomyocytes. of the genes in were to are to of into the of the genes in were to the of to were into cardiomyocytes. the cardiomyocytes. are to of Cbx1, PurB, Cbx1, PurB, an in during of we that to a in the of Cbx1, PurB, To that the of Cbx1, PurB, of a of we the of a during of during to we that in into cardiomyocytes. an of a during of the the the that were to the of our the Cbx1, PurB, were in were in the in of were in the the were during to in PurB, fibroblasts to into in to Cbx1, PurB, genes in in of the three proteins to to fibroblasts in protein gene to Cbx1, PurB, ","journal":"Journal of Biological Chemistry","year":2022,"id":275291,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.951,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":363554,"name":"Richard E. Pratt","orcid":"0000-0002-8725-6249","position":1,"is_corresponding":false},{"id":537806,"name":"José A. Gómez","orcid":"0000-0001-8148-8308","position":2,"is_corresponding":false},{"id":3649,"name":"Victor J. Dzau","orcid":"0000-0002-8280-519X","position":3,"is_corresponding":false},{"id":363555,"name":"Conrad P. Hodgkinson","orcid":"0000-0002-6460-7333","position":4,"is_corresponding":false},{"id":936398,"name":"Syeda S. Baksh","orcid":null,"position":0,"is_corresponding":true}],"reference_count":37,"raw_metadata":null,"created_at":"2026-07-19T00:28:16.928746Z","pmid":"35605661","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}