{"doi":"10.1016/j.jacig.2023.100131","title":"Eosinophil trafficking in allergen-mediated pulmonary inflammation relies on IL-13–driven CCL-11 and CCL-24 production by tissue fibroblasts and myeloid cells","abstract":"Background The immunologic mechanisms underlying pulmonary type 2 inflammation, including the dynamics of eosinophil recruitment to the lungs, still need to be elucidated. Objective We sought to investigate how IL-13–producing T H 2 effector cells trigger eosinophil migration in house dust mite (HDM)-driven allergic pulmonary inflammation. Methods Multiparameter and molecular profiling of murine lungs with HDM-induced allergy was investigated in the absence of IL-13 signaling by using IL-13Rα1–deficient mice and separately through adoptive transfer of CD4 + T cells from IL-5–deficient mice into TCRα –/– mice before allergic inflammation. Results We demonstrated through single-cell techniques that HDM-driven pulmonary inflammation displays a profile characterized by T H 2 effector cell–induced IL-13–dominated eosinophilic inflammation. Using HDM-sensitized IL-13Rα1 –/– mice, we found a marked reduction in the influx of eosinophils into the lungs along with a significant downregulation of both CCL-11 and CCL-24. We further found that eosinophil trafficking to the lung relies on production of IL-13–driven CCL-11 and CCL-24 by fibroblasts and Ly6C + (so-called classical) monocytes. Moreover, this IL-13–mediated eotaxin-dependent eosinophil influx to the lung tissue required IL-5–induced eosinophilia. Finally, we demonstrated that this IL-13–driven eosinophil-dominated pulmonary inflammation was critical for limiting bystander lung transiting Ascaris parasites in a model of allergy and helminth interaction. Conclusion Our data suggest that IL-5–dependent allergen-specific T H 2 effector cell response and subsequent signaling through the IL-13/IL-13Rα1 axis in fibroblasts and myeloid cells regulate the eotaxin-dependent recruitment of eosinophils to the lungs, with multiple downstream consequences, including bystander control of lung transiting parasitic helminths.","journal":"Journal of Allergy and Clinical Immunology Global","year":2023,"id":338533,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9541,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":673841,"name":"Dominic P. Golec","orcid":"0000-0002-2741-3302","position":1,"is_corresponding":false},{"id":794755,"name":"Erik P. Karmele","orcid":null,"position":2,"is_corresponding":false},{"id":718153,"name":"Joshua Sciurba","orcid":"0000-0002-2499-1713","position":3,"is_corresponding":false},{"id":1071411,"name":"Pablo Bara-Garcia","orcid":null,"position":4,"is_corresponding":false},{"id":376615,"name":"Tom Hill","orcid":"0000-0002-4661-6391","position":5,"is_corresponding":false},{"id":287789,"name":"Byunghyun Kang","orcid":"0000-0002-7935-755X","position":6,"is_corresponding":false},{"id":289402,"name":"Sasisekhar Bennuru","orcid":"0000-0002-6117-742X","position":7,"is_corresponding":false},{"id":245103,"name":"Pamela L. Schwartzberg","orcid":"0000-0001-6318-9187","position":8,"is_corresponding":false},{"id":287794,"name":"Thomas B. Nutman","orcid":"0000-0001-6887-4941","position":9,"is_corresponding":false},{"id":1071002,"name":"Pedro H. Gazzinelli-Guimaraes","orcid":"0000-0003-0669-1642","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":null,"created_at":"2026-07-19T01:10:35.973121Z","pmid":"37781651","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}