{"doi":"10.1016/j.isci.2023.106560","title":"Optogenetic activation of UCP1-dependent thermogenesis in brown adipocytes","abstract":"Brown adipocytes are unique in that they expend energy and produce heat to maintain euthermia through expression of uncoupling protein-1 (UCP1). Given their propensity to stimulate weight loss and promote resistance to obesity, they are a compelling interventional target for obesity-related disorders. Here, we tested whether an optogenetic approach could be used to activate UCP1-dependent thermogenesis in brown adipocytes. We generated brown adipocytes expressing a bacterial-derived photoactivatable adenylyl cyclase (bPAC) that, upon blue light stimulation, increases UCP1 expression, fuel uptake and thermogenesis. This unique system allows for precise, chemical free, temporal control of UCP1-dependent thermogenesis, which can aid in our understanding of brown adipocyte biology and development of therapies that target obesity-related disorders.","journal":"iScience","year":2023,"id":350994,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9562,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1095342,"name":"Daniel Nguyen","orcid":"0000-0002-9268-0300","position":1,"is_corresponding":false},{"id":1095725,"name":"Davide Barteselli","orcid":null,"position":2,"is_corresponding":false},{"id":1095726,"name":"Sophia Blanchard","orcid":null,"position":3,"is_corresponding":false},{"id":1095727,"name":"Masen Pelletier","orcid":null,"position":4,"is_corresponding":false},{"id":1095343,"name":"Devesh Kesharwani","orcid":"0000-0001-6940-3703","position":5,"is_corresponding":false},{"id":1095728,"name":"Ed Jachimowicz","orcid":null,"position":6,"is_corresponding":false},{"id":383488,"name":"Su Su","orcid":"0000-0002-8582-795X","position":7,"is_corresponding":false},{"id":580920,"name":"Michele Karolak","orcid":null,"position":8,"is_corresponding":false},{"id":1095344,"name":"Aaron C. Brown","orcid":"0000-0003-0827-2337","position":9,"is_corresponding":false},{"id":1095724,"name":"Chad C. Doucette","orcid":null,"position":0,"is_corresponding":true}],"reference_count":44,"raw_metadata":null,"created_at":"2026-07-19T01:12:37.409066Z","pmid":"37123235","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}