{"doi":"10.1016/j.isci.2023.105992","title":"Chrna5 and lynx prototoxins identify acetylcholine super-responder subplate neurons","abstract":"Attention depends on cholinergic excitation of prefrontal neurons but is sensitive to perturbation of α5-containing nicotinic receptors encoded by Chrna5 . However, Chrna5 -expressing (Chrna5+) neurons remain enigmatic, despite their potential as a target to improve attention. Here, we generate complex transgenic mice to probe Chrna5+ neurons and their sensitivity to endogenous acetylcholine. Through opto-physiological experiments, we discover that Chrna5+ neurons contain a distinct population of acetylcholine super-responders. Leveraging single-cell transcriptomics, we discover molecular markers conferring subplate identity on this subset. We determine that Chrna5+ super-responders express a unique complement of GPI-anchored lynx prototoxin genes ( Lypd1 , Ly6g6e, and Lypd6b ), predicting distinct nicotinic receptor regulation. To manipulate lynx regulation of endogenous nicotinic responses, we developed a pharmacological strategy guided by transcriptomic predictions. Overall, we reveal Chrna5- Cre mice as a transgenic tool to target the diversity of subplate neurons in adulthood, yielding new molecular strategies to manipulate their cholinergic activation relevant to attention disorders.","journal":"iScience","year":2023,"id":333602,"datarank":0.47032413238937254,"base_score":3.1354942159291497,"endowment":3.1354942159291497,"self_citation_contribution":0.47032413238937254,"citation_network_contribution":0.0,"self_endowment_contribution":0.47032413238937254,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9596,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1061379,"name":"Tianhui Chen","orcid":"0000-0003-2236-2224","position":1,"is_corresponding":false},{"id":353434,"name":"Yupeng Liu","orcid":"0000-0001-8347-7338","position":2,"is_corresponding":false},{"id":467656,"name":"Eric E. Turner","orcid":"0000-0001-5499-6788","position":3,"is_corresponding":false},{"id":560534,"name":"Shreejoy J. Tripathy","orcid":"0000-0002-1007-9061","position":4,"is_corresponding":false},{"id":1061380,"name":"Evelyn K. Lambe","orcid":"0000-0002-5994-6090","position":5,"is_corresponding":false},{"id":1061378,"name":"Sridevi Venkatesan","orcid":"0000-0001-9175-7161","position":0,"is_corresponding":true}],"reference_count":102,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:09:44.297718Z","pmid":"36798433","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}