{"doi":"10.1016/j.intimp.2023.110199","title":"Fasudil compensates podocyte injury via CaMK4/Rho GTPases signal and actin cytoskeleton-dependent activation of YAP in MRL/lpr mice","abstract":null,"journal":"International Immunopharmacology","year":2023,"id":672196,"datarank":0.3453877639491069,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.0,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":279635,"name":"Shuo Huang","orcid":"0000-0003-3055-7427","position":1,"is_corresponding":false},{"id":1756224,"name":"Wangda Xu","orcid":null,"position":2,"is_corresponding":false},{"id":1746419,"name":"Guanqun Xie","orcid":null,"position":3,"is_corresponding":false},{"id":1756225,"name":"Yihong Gan","orcid":null,"position":4,"is_corresponding":false},{"id":1746417,"name":"Fugang Huang","orcid":null,"position":5,"is_corresponding":false},{"id":1746420,"name":"Yongsheng Fan","orcid":null,"position":6,"is_corresponding":false},{"id":987517,"name":"Jie Bao","orcid":"0000-0002-1993-7309","position":7,"is_corresponding":false},{"id":1756223,"name":"Fengyuan Tian","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Fasudil compensates podocyte injury via CaMK4/Rho GTPases signal and actin cytoskeleton-dependent activation of YAP in MRL/lpr mice","abstract":"Deposition of immune complexes in the glomerulus leads to irreversible renal damage in lupus nephritis (LN), of which podocyte malfunction arises earlier. Fasudil, the only Rho GTPases inhibitor approved in clinical settings, possesses well-established renoprotective actions; yet, no studies addressed the amelioration derived from fasudil in LN. To clarify, we investigated whether fasudil exerted renal remission in lupus-prone mice. In this study, fasudil (20 mg/kg) was intraperitoneally administered to female MRL/lpr mice for 10 weeks. We report that fasudil administration swept antibodies (anti-dsDNA) and attenuated systemic inflammatory response in MRL/lpr mice, accompanied by preserving podocyte ultrastructure and averting immune complex deposition. Mechanistically, it repressed the expression of CaMK4 in glomerulopathy by preserving nephrin and synaptopodin expression. And fasudil further blocked cytoskeletal breakage in the Rho GTPases-dependent action. Further analyses showed that beneficial actions of fasudil on the podocytes required intra-nuclear YAP activation underlying actin dynamics. In addition, in vitro assays revealed that fasudil normalized the motile imbalance by suppressing intracellular calcium enrichment, thereby contributing to the resistance of apoptosis in podocytes. Altogether, our findings suggest that the precise manners of crosstalks between cytoskeletal assembly and YAP activation underlying the upstream CaMK4/Rho GTPases signal in podocytes is a reliable target for podocytopathies treatment, and fasudil might serve as a promising therapeutic agent to compensate for the podocyte injury in LN.","is_dataset_classified":null,"base_score":2.302585092994046,"endowment":2.302585092994046,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"37094544","pmcid":null,"openalex_id":"https://openalex.org/W4366834443","authors":[],"funders":[{"funder_name":"Natural Science Foundation of Zhejiang Province","grant_id":"LY20H270005","title":null},{"funder_name":"Zhejiang Chinese Medical University","grant_id":"2021JKZKTS012B","title":null},{"funder_name":"Zhejiang Chinese Medical University","grant_id":"BZXCG-2022-21","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"81803980","title":null}],"total_grants":4,"fwci":1.3618,"citation_percentile":0.7958436,"influential_citations":0,"citation_trend":[{"year":2023,"count":1},{"year":2024,"count":6},{"year":2025,"count":2}],"oa_status":"closed","license":"https://doi.org/10.15223/policy-004","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S1567576923005209?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1567576923005209?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.intimp.2023.110199","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/37094544","host_type":"repository"}],"fields_of_study":["Renal Diseases and Glomerulopathies","Erythrocyte Function and Pathophysiology","Hippo pathway signaling and YAP/TAZ","Female","Mice","Animals","Podocytes","rho GTP-Binding Proteins","Mice, Inbred MRL lpr","Lupus Nephritis","Actin Cytoskeleton","Calcium-Calmodulin-Dependent Protein Kinase Type 4","1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine"],"mesh_terms":["Animals","Female","Lupus Nephritis","Actin Cytoskeleton","1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine","Mice, Inbred MRL lpr","rho GTP-Binding Proteins","Podocytes","Mice","Calcium-Calmodulin-Dependent Protein Kinase Type 4"],"keywords":["Fasudil","Podocyte","Cell biology","Podocin","Podocalyxin","RHOA","Actin cytoskeleton","GTPase","Rho kinase inhibitor","Rho-associated protein kinase","Cytoskeleton","Chemistry","Pharmacology","Signal transduction","Cancer research","Medicine","Biology","Internal medicine","Kidney","Biochemistry","Cell","Lupus nephritis","Calcium signaling","Yes-associated Protein (Yap)"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T08:01:51.215715Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}