{"doi":"10.1016/j.immuni.2021.08.026","title":"Conventional type I dendritic cells maintain a reservoir of proliferative tumor-antigen specific TCF-1+ CD8+ T cells in tumor-draining lymph nodes","abstract":"In tumors, a subset of CD8<sup>+</sup> T cells expressing the transcription factor TCF-1 drives the response to immune checkpoint blockade. We examined the mechanisms that maintain these cells in an autochthonous model of lung adenocarcinoma. Longitudinal sampling and single-cell sequencing of tumor-antigen specific TCF-1<sup>+</sup> CD8<sup>+</sup> T cells revealed that while intratumoral TCF-1<sup>+</sup> CD8<sup>+</sup> T cells acquired dysfunctional features and decreased in number as tumors progressed, TCF-1<sup>+</sup> CD8<sup>+</sup> T cell frequency in the tumor draining LN (dLN) remained stable. Two discrete intratumoral TCF-1<sup>+</sup> CD8<sup>+</sup> T cell subsets developed over time-a proliferative SlamF6<sup>+</sup> subset and a non-cycling SlamF6<sup>-</sup> subset. Blocking dLN egress decreased the frequency of intratumoral SlamF6<sup>+</sup> TCF-1<sup>+</sup> CD8<sup>+</sup> T cells. Conventional type I dendritic cell (cDC1) in dLN decreased in number with tumor progression, and Flt3L+anti-CD40 treatment recovered SlamF6<sup>+</sup> T cell frequencies and decreased tumor burden. Thus, cDC1s in tumor dLN maintain a reservoir of TCF-1<sup>+</sup> CD8<sup>+</sup> T cells and their decrease contributes to failed anti-tumor immunity.","journal":"Immunity","year":2021,"id":6717,"datarank":0.8386480470766287,"base_score":5.5909869805108565,"endowment":5.5909869805108565,"self_citation_contribution":0.8386480470766287,"citation_network_contribution":0.0,"self_endowment_contribution":0.8386480470766287,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":267,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.0469,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-10-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":17248,"name":"Rebecca H. 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