{"doi":"10.1016/j.ijid.2023.03.050","title":"Evolution of Plasmodium falciparum antimalarial drug resistance markers post-adoption of artemisinin-based combination therapies in Yaounde, Cameroon","abstract":"OBJECTIVES: This study aimed to investigate the evolution of Plasmodium falciparum antimalarial drug resistance markers by comparing the pre- and post-adoption of artemisinin-based combination therapies (ACTs) in Yaounde, Cameroon. METHODS: The molecular characterization of known antimalarial drug resistance markers (Pfcrt, Pfmdr1, Pfdhfr, Pfdhps, and Pfk13) in P. falciparum-positive samples collected in 2014 and 2019-2020 was achieved using nested polymerase chain reaction, followed by targeted amplicon deep sequencing on the Illumina MiSeq platform. Data derived were compared with those published during the pre-ACT adoption period from 2004 to 2006. RESULTS: A high prevalence of Pfmdr1 184F, Pfdhfr 51I/59R/108N, and Pfdhps 437G mutant alleles was observed during the post-ACT adoption period. The Pfcrt 76T and Pfmdr1 86Y mutant alleles significantly declined between 2004 and 2020 (P <0.0001). Conversely, the resistance markers to antifolates, Pfdhfr 51I/59R/108N and Pfdhps 437G, significantly increased during the same study period (P <0.0001). We identified nine mutations in the propeller domains of Pfk13; although they were all present in single parasite isolates, none of them are known to confer artemisinin resistance. CONCLUSION: This study documented a near-complete reversion to sensitive parasites for markers conferring resistance to the 4-aminoquinolines and arylamino alcohols in Yaounde. In contrast, the Pfdhfr mutations associated with pyrimethamine resistance are moving toward saturation.","journal":"International Journal of Infectious Diseases","year":2023,"id":333776,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":22,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.782,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1061557,"name":"Akindeh M. Nji","orcid":"0000-0003-0343-1722","position":1,"is_corresponding":false},{"id":1062179,"name":"Jean Paul Kengne Chedjou","orcid":null,"position":2,"is_corresponding":false},{"id":1061558,"name":"Helle Hansson","orcid":"0000-0001-6484-1165","position":3,"is_corresponding":false},{"id":1061559,"name":"Emma Filtenborg Hocke","orcid":"0000-0002-0040-7041","position":4,"is_corresponding":false},{"id":1061560,"name":"Innocent Mbulli Ali","orcid":"0000-0002-1112-6376","position":5,"is_corresponding":false},{"id":1053774,"name":"Olivia Achonduh-Atijegbe","orcid":"0000-0003-3583-522X","position":6,"is_corresponding":false},{"id":1062180,"name":"Marie-Solange B. Evehe","orcid":null,"position":7,"is_corresponding":false},{"id":1062181,"name":"Marie Helene Munck Jørgensen","orcid":null,"position":8,"is_corresponding":false},{"id":1061561,"name":"Calvino Tah Fomboh","orcid":"0000-0002-2730-9847","position":9,"is_corresponding":false},{"id":308914,"name":"Liwang Cui","orcid":"0000-0002-8338-1974","position":10,"is_corresponding":false},{"id":877728,"name":"Gillian Stresman","orcid":"0000-0002-6897-5459","position":11,"is_corresponding":false},{"id":436314,"name":"Jude D. Bigoga","orcid":"0000-0001-7206-3308","position":12,"is_corresponding":false},{"id":940302,"name":"Michael Alifrangis","orcid":"0000-0002-3835-3333","position":13,"is_corresponding":false},{"id":436313,"name":"Wilfred Fon Mbacham","orcid":"0000-0002-3934-3233","position":14,"is_corresponding":false},{"id":1061556,"name":"Peter Thelma Ngwa Niba","orcid":"0000-0001-8414-8202","position":0,"is_corresponding":true}],"reference_count":27,"raw_metadata":null,"created_at":"2026-07-19T01:09:44.297718Z","pmid":"37028468","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}