{"doi":"10.1016/j.ijid.2008.05.1338","title":"Molecular Diagnosis of Common Viral Infectious Diseases Based on Real Time PCR","abstract":null,"journal":"International Journal of Infectious Diseases","year":2008,"id":683033,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1784358,"name":"J. Bloomberg","orcid":null,"position":1,"is_corresponding":false},{"id":1784357,"name":"N. Mohamed","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Molecular Diagnosis of Common Viral Infectious Diseases Based on Real Time PCR","abstract":"Molecular biology has become an integral part of the diagnosis of infectious diseases. Recently, quantitative real-time PCR (QPCR) methods have been developed for the diagnosis of a wide range of infectious diseases. The use of QPCR as a tool for diagnostic virological and viral research laboratories has greatly increased in recent years. It often replaces conventional PCR and amplicon detection systems which are more complex and laborious, with a higher risk of amplicon carry-over contamination. The new QPCR methods presented here utilize broadly targeted primers and probes for rational and sensitive detection and quantification of variable RNA viruses. They take advantage of the dual properties, both RNA and DNA dependent DNA polymerase activities, of the rTth thermostable polymerase, and thermolabile UNG with dUTP to protect against inadvertent contamination of samples with amplimers. Specimen collection and delivery to the laboratory without delay and with minimal losses are vital components for reliable RNA measurements in a diagnostic service. 62 Cerebrospinal fluid, 71 stool specimens & 203 human nasopharyngeal aspirates involved in this study. We describe broadly targeted QPCRs which use several alternative conserved sequences. The assays developed will be useful tools for performing infection surveys. The development of broadly reactive QPCRs assays for RNA viruses was challenging due to the extensive genetic diversity among the different viruses. The assays in the present study were able to detect all or most of the intended target viruses in less than 3 hours, to achieve the ideals broad targeting, high sensitivity, high specificity and quantitative results. The technologies described in this study have common features. They are a platform for further development of diagnostic tools for screening and detection of viruses in known viral diseases, maybe also for discovering new viruses.","is_dataset_classified":null,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21097893","pmcid":null,"openalex_id":"https://openalex.org/W2014035551","authors":[],"funders":[],"total_grants":0,"fwci":0.0,"citation_percentile":0.16473876,"influential_citations":0,"citation_trend":[{"year":2015,"count":1},{"year":2022,"count":1}],"oa_status":"gold","license":"https://www.elsevier.com/open-access/userlicense/1.0/","oa_locations":[{"url":"http://www.ijidonline.com/article/S1201971208016524/pdf","host_type":"journal"},{"url":"http://www.ijidonline.com/article/S1201971208016524/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1201971208016524?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1201971208016524?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.ijid.2008.05.1338","host_type":"journal"},{"url":"http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7118","host_type":"repository"}],"fields_of_study":["Animal Disease Management and Epidemiology","Viral Infections and Immunology Research","Viral gastroenteritis research and epidemiology"],"mesh_terms":[],"keywords":["Amplicon","Biology","Virology","Molecular diagnostics","Polymerase chain reaction","Computational biology","Real-time polymerase chain reaction","Genetics","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T22:06:18.586467Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}