{"doi":"10.1016/j.heliyon.2018.e00633","title":"Development of norepinephrine transporter reuptake inhibition assays using SK-N-BE(2)C cells","abstract":null,"journal":"Heliyon","year":2018,"id":625642,"datarank":0.24141568686511508,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.0,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":356298,"name":"Bruce E. Blough","orcid":"0000-0003-3515-0896","position":1,"is_corresponding":false},{"id":505301,"name":"Ann M. Decker","orcid":"0000-0001-7508-2096","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Development of norepinephrine transporter reuptake inhibition assays using SK-N-BE(2)C cells","abstract":"This report describes efforts to develop and validate novel norepinephrine transporter reuptake inhibition assays using human neuroblastoma SK-N-BE(2)C cells in 24-well format. Before conducting the assays, the SK-N-BE(2)C cells were first evaluated for their ability to uptake [<sup>3</sup>H]norepinephrine and were shown to have a saturable uptake with a K<sub>M</sub> value of 416 nM. Using this determined K<sub>M</sub> value, reuptake inhibition assays were then conducted with a variety of ligands including antidepressants, as well as piperazine and phenyltropane derivatives. The results obtained with the SK-N-BE(2)C cells indicate that this model system can detect a range of ligand potencies, which compare well with other established transporter assays. Our data suggest that SK-N-BE(2)C cells have potential utility to serve as another model system to detect norepinephrine reuptake inhibition activity.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"29872766","pmcid":"PMC5986547","openalex_id":null,"authors":[],"funders":[{"funder_name":"National Institutes of Health","grant_id":"R03DA036208","title":null},{"funder_name":"National Institutes of Health","grant_id":"1R03DA036208-01A1","title":"Development of Cell-Based Human Dopamine, Norepinephrine, and Serotonin Transporter Release Assays"},{"funder_name":"National Institute on Drug Abuse","grant_id":"","title":null}],"total_grants":3,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"gold","license":"cc-by-nc-nd","oa_locations":[{"url":"http://www.cell.com/article/S2405844018316062/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2405844018316062?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2405844018316062?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doaj.org/article/0270d616d6834fc68f1efd94e0d17bc7","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC5986547","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC5986547?pdf=render","host_type":"Europe_PMC"},{"url":"https://doi.org/10.1016/j.heliyon.2018.e00633","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/29872766","host_type":""},{"url":"http://dx.doi.org/10.1016/j.heliyon.2018.e00633","host_type":""},{"url":"https://dx.doi.org/10.1016/j.heliyon.2018.e00633","host_type":""}],"fields_of_study":["0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":[],"keywords":["Neuroscience","Social sciences (General)","H1-99","Q1-390","Science (General)","Article"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. Good health"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-04T07:22:01.430866Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}