{"doi":"10.1016/j.gene.2021.145969","title":"RNA-seq analysis reveals the positive role of KLF5 in the differentiation of subcutaneous adipocyte in goats","abstract":null,"journal":"Gene","year":2022,"id":601917,"datarank":0.42498200160843247,"base_score":2.833213344056216,"endowment":2.833213344056216,"self_citation_contribution":0.42498200160843247,"citation_network_contribution":0.0,"self_endowment_contribution":0.42498200160843247,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":589699,"name":"Hao Zhang","orcid":"0000-0002-9192-4684","position":1,"is_corresponding":false},{"id":755728,"name":"Yong Wang","orcid":"0000-0002-8349-7555","position":2,"is_corresponding":false},{"id":711472,"name":"Yanyan Li","orcid":"0009-0005-7231-9293","position":3,"is_corresponding":false},{"id":1543488,"name":"Changsheng He","orcid":null,"position":4,"is_corresponding":false},{"id":293945,"name":"Jiangjiang Zhu","orcid":"0000-0002-4548-8949","position":5,"is_corresponding":false},{"id":1026599,"name":"Yan Xiong","orcid":"0000-0001-5507-6991","position":6,"is_corresponding":false},{"id":1543489,"name":"Yaqiu Lin","orcid":null,"position":7,"is_corresponding":false},{"id":801787,"name":"Xin Li","orcid":"0000-0002-8779-125X","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"RNA-seq analysis reveals the positive role of KLF5 in the differentiation of subcutaneous adipocyte in goats","abstract":"As the largest energy storage reservoir, subcutaneous adipose tissue (SAT) stores excess lipids by adipocytes enlargement and/or recruitment of new precursor cells. Energy overload can cause ectopic fat deposition and metabolic diseases. In this study, 6814 differentially expressed genes (DEGs) were screened in goat subcutaneous preadipocytes and mature adipocytes by RNA-seq technique. The relative expression of the DEGs were verified by qPCR, such as PLIN2, MECR, ADCY7, PEBP1 and KLF5, and their expression level was found to be consistent with the trend of RNA-seq analysis. The KLF5 was selected for further functional verification. Overexpression of KLF5 promoted both the adipogenesis and the differentiation of preadipocytes, while the expression of preadipocyte marker gene: preadipocyte factor 1(Pref1) was decreased, as well as, peroxisome proliferator activation Receptor γ(PPARγ), CCAAT enhancer binding protein β(C/EBPβ) and Sterol regulatory element binding protein isoform 1(SREBP1) were increased. On the contrary, the interference of KLF5 could reduce adipogenesis, enhance the expression of Pref1, and reduce the expression of C/EBPβ and SREBP1. Our research provides a basic reference for revealing the mechanism of subcutaneous adipocyte differentiation in goats.","is_dataset_classified":null,"base_score":2.833213344056216,"endowment":2.833213344056216,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34530084","pmcid":null,"openalex_id":"https://openalex.org/W3201371670","authors":[],"funders":[],"total_grants":0,"fwci":1.0365,"citation_percentile":0.75474793,"influential_citations":0,"citation_trend":[{"year":2022,"count":4},{"year":2023,"count":5},{"year":2024,"count":5},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":"https://doi.org/10.15223/policy-004","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0378111921005643?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0378111921005643?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.gene.2021.145969","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/34530084","host_type":"repository"}],"fields_of_study":["Kruppel-like factors research","Peroxisome Proliferator-Activated Receptors","Adipose Tissue and Metabolism","Adipocytes","Adipogenesis","Animals","Cell Differentiation","Gene Expression","Gene Expression Profiling","Gene Expression Regulation","Goats","Kruppel-Like Transcription Factors","RNA-Seq","Sequence Analysis, RNA","Subcutaneous Fat","Transcriptional Activation","Transcriptome"],"mesh_terms":["RNA-Seq","Animals","Cell Differentiation","Gene Expression Regulation","Goats","Transcriptional Activation","Gene Expression","Sequence Analysis, RNA","Adipocytes","Gene Expression Profiling","Subcutaneous Fat","Adipogenesis","Kruppel-Like Transcription Factors","Transcriptome"],"keywords":["Adipogenesis","Biology","Adipocyte","Adipose tissue","Internal medicine","Ectopic expression","Endocrinology","Gene expression","Sterol regulatory element-binding protein","Gene isoform","Peroxisome proliferator-activated receptor","Peroxisome","Transcription factor","Cell biology","Gene","Molecular biology","Biochemistry","Differentiation","Goat","Rna-seq","Klf5","Subcutaneous Adipocyte"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Affordable and clean energy"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T17:46:31.656717Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}