{"doi":"10.1016/j.gene.2020.144813","title":"Challenges of in vitro genome editing with CRISPR/Cas9 and possible solutions: A review","abstract":null,"journal":"Gene","year":2020,"id":590667,"datarank":1.013667608925997,"base_score":3.4339872044851463,"endowment":3.4339872044851463,"self_citation_contribution":0.515098080672772,"citation_network_contribution":0.4985695282532252,"self_endowment_contribution":0.515098080672772,"citer_contribution":0.4985695282532252,"corpus_percentile":null,"corpus_rank":null,"citation_count":30,"citer_count":27,"citers_with_citation_signal":25,"citers_with_endowment":25,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1511261,"name":"Atieh Hashemi","orcid":"0000-0001-7121-5306","position":1,"is_corresponding":false},{"id":1511260,"name":"Vida Ebrahimi","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Challenges of in vitro genome editing with CRISPR/Cas9 and possible solutions: A review","abstract":"Microbial production of bio-based ingredients often requires metabolically engineered bacterial strains with the edited genome. Genome editing tools are also essential for gene identification and investigating genotype-phenotype connections. Currently, one of the most common tools of genome editing is based on a natural bacterial adaptive immune system known as CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 (CRISPR-associated protein 9) due to its simple, rapid, and efficient activities. Although successful in some in vitro systems, its application as an approach of metabolic engineering and genome editing is still not so extensive. Here, we discuss existing barriers and challenges of the CRISPR/Cas9 editing tool for in vitro systems. Firstly, we aim to briefly introduce the CRISPR/Cas9 method as an in vitro gene editing tool. Next, we discuss existing obstacles to CRISPR-based editing in bacterial and in vitro model systems and offer guidelines to help achieve editing in an expanded range of in vitro systems.","is_dataset_classified":null,"base_score":3.4339872044851463,"endowment":3.4339872044851463,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"32470504","pmcid":null,"openalex_id":"https://openalex.org/W3030388918","authors":[],"funders":[],"total_grants":0,"fwci":1.1928,"citation_percentile":0.77612656,"influential_citations":1,"citation_trend":[{"year":2020,"count":4},{"year":2021,"count":4},{"year":2022,"count":7},{"year":2023,"count":1},{"year":2024,"count":6},{"year":2025,"count":7},{"year":2026,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/legal/tdmrep-license","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0378111920304820?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0378111920304820?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.gene.2020.144813","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/32470504","host_type":"repository"}],"fields_of_study":["CRISPR and Genetic Engineering","Bacterial Genetics and Biotechnology","Virus-based gene therapy research","Medicine","Biology","Animals","Bacteria","Bacterial Proteins","CRISPR-Associated Protein 9","CRISPR-Cas Systems","Clustered Regularly Interspaced Short Palindromic Repeats","Gene Editing","Genome, Bacterial","Humans","Metabolic Engineering"],"mesh_terms":["Gene Editing","CRISPR-Associated Protein 9","Animals","Bacteria","Bacterial Proteins","Humans","Genome, Bacterial","Metabolic Engineering","Clustered Regularly Interspaced Short Palindromic Repeats","CRISPR-Cas Systems"],"keywords":["CRISPR","Genome editing","Biology","Cas9","Computational biology","Genome","Synthetic biology","Gene","Genetics","Bacteria","Challenges","Crispr/cas9","In Vitro Model Systems"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-25T06:56:55.093950Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}