{"doi":"10.1016/j.gene.2006.05.027","title":"Tab2, a novel recombinant polypeptide tag offering sensitive and specific protein detection and reliable affinity purification","abstract":null,"journal":"Gene","year":2006,"id":607830,"datarank":0.26876392038420827,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.0,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1560838,"name":"Silke Finster","orcid":null,"position":1,"is_corresponding":false},{"id":1560839,"name":"John McClary","orcid":null,"position":2,"is_corresponding":false},{"id":134097,"name":"Wei Xia","orcid":null,"position":3,"is_corresponding":false},{"id":1560840,"name":"Brent Larsen","orcid":null,"position":4,"is_corresponding":false},{"id":1560842,"name":"Douglas Schneider","orcid":null,"position":5,"is_corresponding":false},{"id":1560844,"name":"Hong-Tao Lu","orcid":null,"position":6,"is_corresponding":false},{"id":1560846,"name":"Sara Biancalana","orcid":null,"position":7,"is_corresponding":false},{"id":1560847,"name":"Jian-Ai Xuan","orcid":null,"position":8,"is_corresponding":false},{"id":1560848,"name":"Alicia Newton","orcid":null,"position":9,"is_corresponding":false},{"id":1560849,"name":"Debbie Allen","orcid":null,"position":10,"is_corresponding":false},{"id":1560850,"name":"Peter Bringmann","orcid":null,"position":11,"is_corresponding":false},{"id":618427,"name":"Ronald R. Cobb","orcid":"0000-0002-3506-8123","position":12,"is_corresponding":false},{"id":1560837,"name":"Kerstin Crusius","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Tab2, a novel recombinant polypeptide tag offering sensitive and specific protein detection and reliable affinity purification","abstract":"The detection and purification of proteins are often time-consuming and frequently involve complicated protocols. The addition of a peptide tag to recombinant proteins can make this process more efficient. Many of the commonly used tags, such as Flagtrade mark, Myc, HA and V5 are recognized by specific monoclonal antibodies and therefore, allow immunoaffinity-based purification. Enhancing the current scope of flexibility in using diverse peptide tags, we report here the development of a novel, short polypeptide tag (Tab2) for detection and purification of recombinant proteins. The Tab2 epitope corresponds to the NH2-terminal seven amino acid residues of human TGFalpha. A monoclonal anti-Tab2 antibody was raised and characterized. To investigate the potential of this peptide sequence as a novel tag for recombinant proteins, we expressed several different recombinant proteins containing this tag in E. coli, baculovirus, and mammalian cells. The data presented demonstrates the Tab2 tag-anti-Tab2 antibody combination is a reliable tool enabling specific Western blot detection, FACS analysis, and immunoprecipitation as well as non-denaturing protein affinity purification.","is_dataset_classified":null,"base_score":1.791759469228055,"endowment":1.791759469228055,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16879932","pmcid":null,"openalex_id":"https://openalex.org/W2072449934","authors":[],"funders":[],"total_grants":0,"fwci":0.4633,"citation_percentile":0.65300978,"influential_citations":0,"citation_trend":[{"year":2013,"count":2}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0378111906003477?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0378111906003477?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.gene.2006.05.027","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16879932","host_type":"repository"}],"fields_of_study":["Peptidase Inhibition and Analysis","Monoclonal and Polyclonal Antibodies Research","Cell Adhesion Molecules Research","Amino Acid Motifs","Animals","Antibodies, Monoclonal","Antibody Specificity","Cells, Cultured","Chromatography, Affinity","Cloning, Molecular","Enzyme-Linked Immunosorbent Assay","Epitopes","Flow Cytometry","Genetic Vectors","Humans","Immunoprecipitation","Insecta","Peptide Fragments","Phosphotransferases","Protein Binding","Protein Structure, Tertiary","Receptors, Cell Surface","Recombinant Fusion Proteins","Transforming Growth Factor alpha"],"mesh_terms":["Animals","Antibodies, Monoclonal","Antibody Specificity","Epitopes","Cells, Cultured","Chromatography, Affinity","Cloning, Molecular","Enzyme-Linked Immunosorbent Assay","Flow Cytometry","Genetic Vectors","Humans","Insecta","Peptide Fragments","Phosphotransferases","Protein Binding","Receptors, Cell Surface","Recombinant Fusion Proteins","Transforming Growth Factor alpha","Protein Structure, Tertiary","Amino Acid Motifs","Immunoprecipitation"],"keywords":["Recombinant DNA","Myc-tag","FLAG-tag","Protein tag","Biology","Monoclonal antibody","Immunoprecipitation","Epitope","Molecular biology","Peptide","Affinity chromatography","Biochemistry","Antibody","Computational biology","Fusion protein","Gene","Enzyme","Genetics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T07:04:25.733933Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}