{"doi":"10.1016/j.fsi.2022.01.029","title":"hnRNPub inhibits LPS-induced NF-κB pathway by targeting TRAF6 for K48-linked ubiquitination in miiuy croaker (Miichthys miiuy)","abstract":null,"journal":"Fish & Shellfish Immunology","year":2022,"id":667239,"datarank":0.38474240361923057,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"self_citation_contribution":0.38474240361923057,"citation_network_contribution":0.0,"self_endowment_contribution":0.38474240361923057,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":16,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":174518,"name":"Ya Chen","orcid":null,"position":1,"is_corresponding":false},{"id":1742493,"name":"Xiaolong Yan","orcid":null,"position":2,"is_corresponding":false},{"id":933805,"name":"Qi Li","orcid":"0000-0001-5699-9843","position":3,"is_corresponding":false},{"id":39919,"name":"Pengfei Wang","orcid":null,"position":4,"is_corresponding":false},{"id":949755,"name":"Yuena Sun","orcid":null,"position":5,"is_corresponding":false},{"id":949295,"name":"Tianjun Xu","orcid":"0000-0003-3606-8069","position":6,"is_corresponding":false},{"id":717699,"name":"Yang Chen","orcid":"0000-0003-4496-7849","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"hnRNPub inhibits LPS-induced NF-κB pathway by targeting TRAF6 for K48-linked ubiquitination in miiuy croaker (Miichthys miiuy)","abstract":"As an important adaptor protein in innate immunity, TRAF6 is not only responsible for the transduction of signal pathways, but its E3 ligase activity to transfer ubiquitination has also been widely studied. Under LPS stimulation, TRAF6 transfers the K63-linked ubiquitination chain to TAK1, which in turn activates the transcription factor NF-κB and cell signaling factors downstream of the signaling pathway. However, how TRAF6 expression is regulated remains largely unknown, especially in teleost. In this study, we identified hnRNPub as a suppressor of TRAF6 expression. The mRNA level of hnRNPub significantly increased under LPS stimulation, and hnRNPub inhibited NF-κB signaling pathway by targeting TRAF6. Knockdown of hnRNPub potentiated inflammatory cytokines, such as TNFα,IL-1β,IL-8. Mechanistically, hnRNPub inhibited NF-κB signaling pathway through mediating K48-linked ubiquitination and proteasomal degradation of TRAF6. Thus, our findings reveal that hnRNPub limits LPS-induced innate activation by promoting K48-linked polyubiquitination and proteasomal degradation of TRAF6.","is_dataset_classified":null,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"datacite_reuse_total":16,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"35074523","pmcid":null,"openalex_id":"https://openalex.org/W4206989812","authors":[],"funders":[{"funder_name":"National Natural Science Foundation of China","grant_id":"31802325","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"31822057","title":null}],"total_grants":2,"fwci":2.2703,"citation_percentile":0.89943864,"influential_citations":0,"citation_trend":[{"year":2023,"count":5},{"year":2024,"count":3},{"year":2025,"count":3},{"year":2026,"count":1}],"oa_status":"closed","license":"https://doi.org/10.15223/policy-004","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S1050464822000389?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1050464822000389?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.fsi.2022.01.029","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/35074523","host_type":"repository"}],"fields_of_study":["NF-κB Signaling Pathways","interferon and immune responses","Immune Response and Inflammation","Medicine","Biology","Environmental Science"],"mesh_terms":["Animals","Lipopolysaccharides","Perciformes","NF-kappa B","Heterogeneous-Nuclear Ribonucleoprotein U","TNF Receptor-Associated Factor 6","Ubiquitination"],"keywords":["Ubiquitin ligase","Ubiquitin","Innate immune system","Signal transduction","Cell biology","Signal transducing adaptor protein","Biology","NF-κB","Gene knockdown","Transcription factor","NFKB1","IκBα","IκB kinase","Stimulation","Receptor","Biochemistry","Gene","Endocrinology","Ubiquitination","Traf6","K48","Hnrnpub"],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.26711558","title":"Additional file 7 of RNA methylation patterns, immune characteristics, and autophagy-related mechanisms mediated by N6-methyladenosine (m6A) regulatory factors in venous thromboembolism","publisher":"figshare","resource_type":"Dataset"},{"doi":"10.6084/m9.figshare.26711555.v1","title":"Additional file 6 of RNA methylation patterns, immune characteristics, and autophagy-related mechanisms mediated by N6-methyladenosine (m6A) regulatory factors in venous 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