{"doi":"10.1016/j.fmre.2025.10.006","title":"Solution landscape of reaction-diffusion systems reveals a nonlinear mechanism and spatial robustness of pattern formation","abstract":"• We propose a generic and efficient numerical algorithm to systematically construct the complete solution landscape of reaction-diffusion systems. • We demonstrate that Turing instability is not the prerequisite to generate stable spatial patterns, uncovering a generic nonlinear mechanism that is beyond the original Turing mechanism. • We elucidate the underlying mechanism for the robust positioning of spatial patterns in a reversible three-species RD system with intrinsic noise. Spontaneous pattern formation in homogeneous systems is ubiquitous in nature. Although Turing demonstrated that spatial patterns can emerge in reaction-diffusion (RD) systems when the homogeneous state becomes linearly unstable, it remains unclear whether Turing mechanism is the only route for pattern formation. Here, we develop an efficient algorithm to systematically construct the solution landscape to find all steady-state solutions connected to a homogeneous state. By applying our method to generic RD models, we find that stable spatial patterns can emerge via saddle-node bifurcations before the onset of Turing instability, and reveal a general nonlinear mechanism that increases the parameter space over which pattern formation occurs in the RD systems. Furthermore, by using a generalized action in the functional space based on large deviation theory, our method is extended to evaluate stability of spatial patterns against noise. Applying this general approach in a three-species RD model, we show that though formation of Turing patterns only requires two chemical species, the third species is critical for stabilizing patterns against strong intrinsic noise in small biochemical systems.","journal":"Fundamental Research","year":2025,"id":539936,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9395,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1427430,"name":"Bing Yu","orcid":"0000-0001-7752-592X","position":1,"is_corresponding":false},{"id":454490,"name":"Yuhai Tu","orcid":"0000-0002-4589-981X","position":2,"is_corresponding":false},{"id":1427431,"name":"Lei Zhang","orcid":"0000-0001-9972-2051","position":3,"is_corresponding":false},{"id":1427429,"name":"Shuonan Wu","orcid":"0000-0003-0578-4097","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:52:34.520788Z","pmid":"39253638","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}