{"doi":"10.1016/j.febslet.2006.09.040","title":"Identification of Hsc70 as an influenza virus matrix protein (M1) binding factor involved in the virus life cycle","abstract":"<jats:p>Influenza virus matrix protein 1 (M1) has been shown to play a crucial role in the virus replication, assembly and budding. We identified heat shock cognate protein 70 (Hsc70) as a M1 binding protein by immunoprecipitation and MALDI‐TOF MS. The C terminal domain of M1 interacts with Hsc70. We found that Hsc70 does not correlate with the transport of M1 to the nucleus, however, it does inhibit the nuclear export of M1 and NP, thus resulting in the inhibition of viral production. This is the first demonstration that Hsc70 is directly associated with M1 and therefore is required for viral production.</jats:p>","journal":"FEBS Letters","year":2006,"id":588742,"datarank":3.8778493611447757,"base_score":4.406719247264253,"endowment":4.406719247264253,"self_citation_contribution":0.6610078870896381,"citation_network_contribution":3.2168414740551374,"self_endowment_contribution":0.6610078870896381,"citer_contribution":3.2168414740551374,"corpus_percentile":null,"corpus_rank":null,"citation_count":81,"citer_count":74,"citers_with_citation_signal":68,"citers_with_endowment":68,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1506255,"name":"Takayuki Fuse","orcid":null,"position":1,"is_corresponding":false},{"id":1506256,"name":"Issay Asano","orcid":null,"position":2,"is_corresponding":false},{"id":1506257,"name":"Fujiko Tsukahara","orcid":null,"position":3,"is_corresponding":false},{"id":1506258,"name":"Yoshiro Maru","orcid":null,"position":4,"is_corresponding":false},{"id":1130675,"name":"Kyosuke Nagata","orcid":"0000-0003-2522-3561","position":5,"is_corresponding":false},{"id":1506259,"name":"Kaio Kitazato","orcid":null,"position":6,"is_corresponding":false},{"id":131442,"name":"Nobuyuki Kobayashi","orcid":null,"position":7,"is_corresponding":false},{"id":1506254,"name":"Ken Watanabe","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Identification of Hsc70 as an influenza virus matrix protein (M1) binding factor involved in the virus life cycle","abstract":"<jats:p>Influenza virus matrix protein 1 (M1) has been shown to play a crucial role in the virus replication, assembly and budding. We identified heat shock cognate protein 70 (Hsc70) as a M1 binding protein by immunoprecipitation and MALDI‐TOF MS. The C terminal domain of M1 interacts with Hsc70. We found that Hsc70 does not correlate with the transport of M1 to the nucleus, however, it does inhibit the nuclear export of M1 and NP, thus resulting in the inhibition of viral production. This is the first demonstration that Hsc70 is directly associated with M1 and therefore is required for viral production.</jats:p>","is_dataset_classified":null,"base_score":4.406719247264253,"endowment":4.406719247264253,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17022977","pmcid":null,"openalex_id":"https://openalex.org/W2079790292","authors":[],"funders":[],"total_grants":0,"fwci":3.0072,"citation_percentile":0.91040963,"influential_citations":0,"citation_trend":[{"year":2012,"count":11},{"year":2013,"count":3},{"year":2014,"count":6},{"year":2015,"count":3},{"year":2016,"count":3},{"year":2017,"count":7},{"year":2018,"count":1},{"year":2019,"count":3},{"year":2020,"count":2},{"year":2021,"count":5},{"year":2022,"count":2},{"year":2023,"count":1},{"year":2024,"count":2},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"bronze","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/j.febslet.2006.09.040","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/j.febslet.2006.09.040","host_type":"publisher"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2Fj.febslet.2006.09.040","host_type":"publisher"},{"url":"https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/j.febslet.2006.09.040","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.febslet.2006.09.040","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17022977","host_type":"repository"}],"fields_of_study":["Influenza Virus Research Studies","Viral Infections and Immunology Research","Heat shock proteins research","Active Transport, Cell Nucleus","Animals","Cell Line","Dogs","HSC70 Heat-Shock Proteins","Humans","Influenza A virus","Protein Binding","Viral Matrix Proteins","Virus Replication"],"mesh_terms":["Animals","Cell Line","Dogs","Humans","Influenza A virus","Protein Binding","Viral Matrix Proteins","Virus Replication","Active Transport, Cell Nucleus","HSC70 Heat-Shock Proteins"],"keywords":["Viral matrix protein","Immunoprecipitation","Virus","Viral life cycle","Viral replication","Influenza A virus","Biology","Virology","VP40","Cell biology","Viral structural protein","Viral protein","Chemistry","Viral entry","Gene","Biochemistry"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Responsible consumption and production"},{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-22T20:04:08.760552Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}