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The interference effect of FMDV IRES was observed in <jats:italic>cis</jats:italic> and <jats:italic>trans</jats:italic>, in support of competition for common transacting factors different than those used in cap‐ and HCV‐dependent initiation.</jats:p>","journal":"FEBS Letters","year":2005,"id":588826,"datarank":0.6485553537266986,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"self_citation_contribution":0.3596842909197557,"citation_network_contribution":0.2888710628069429,"self_endowment_contribution":0.3596842909197557,"citer_contribution":0.2888710628069429,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":6,"citers_with_citation_signal":5,"citers_with_endowment":5,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1506509,"name":"Almudena Pacheco","orcid":null,"position":1,"is_corresponding":false},{"id":1506510,"name":"Jorge Ramajo","orcid":null,"position":2,"is_corresponding":false},{"id":136681,"name":"Sonia López de Quinto","orcid":null,"position":3,"is_corresponding":false},{"id":1504836,"name":"Encarnacion Martinez-Salas","orcid":null,"position":4,"is_corresponding":false},{"id":1506508,"name":"Sandrine Reigadas","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Specific interference between two unrelated internal ribosome entry site elements impairs translation efficiency","abstract":"<jats:p>Internal ribosome entry site (IRES) elements allow simultaneous synthesis of multiple proteins in eukaryotic cells. Here, two unrelated IRESs that perform efficiently in bicistronic constructs, the picornavirus foot‐and‐mouth disease virus (FMDV) and the cellular immunoglobulin heavy chain binding protein (BiP) IRES, were used to generate a tricistronic vector. Functional analysis of the tricistronic RNA evidenced that the efficiency of protein synthesis under the control of BiP IRES was lower than that of the FMDV IRES, relative to the efficiency measured in bicistronic vectors. A specific competition between these elements was verified using two separate mono‐ or bicistronic constructs in vivo and in vitro. In contrast, no interference was detected with the hepatitis C virus (HCV) IRES. The interference effect of FMDV IRES was observed in <jats:italic>cis</jats:italic> and <jats:italic>trans</jats:italic>, in support of competition for common transacting factors different than those used in cap‐ and HCV‐dependent initiation.</jats:p>","is_dataset_classified":null,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16330032","pmcid":null,"openalex_id":"https://openalex.org/W2006571365","authors":[],"funders":[],"total_grants":0,"fwci":1.2212,"citation_percentile":0.81031561,"influential_citations":0,"citation_trend":[{"year":2012,"count":2}],"oa_status":"bronze","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/j.febslet.2005.11.015","host_type":"journal"},{"url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1016/j.febslet.2005.11.015","host_type":"publisher"},{"url":"https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2Fj.febslet.2005.11.015","host_type":"publisher"},{"url":"https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/j.febslet.2005.11.015","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.febslet.2005.11.015","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16330032","host_type":"repository"},{"url":"https://orca.cardiff.ac.uk/id/eprint/1174/","host_type":"repository"}],"fields_of_study":["Viral Infections and Immunology Research","RNA and protein synthesis mechanisms","Viral Infectious Diseases and Gene Expression in Insects","Animals","Cell Line","Cricetinae","Endoplasmic Reticulum Chaperone BiP","Foot-and-Mouth Disease Virus","HeLa Cells","Heat-Shock Proteins","Hepacivirus","Humans","Molecular Chaperones","Peptide Chain Initiation, Translational","Protein Biosynthesis","RNA Caps","RNA, Viral","Ribosomes"],"mesh_terms":["Endoplasmic Reticulum Chaperone BiP","Animals","Cell Line","Cricetinae","Heat-Shock Proteins","HeLa Cells","Humans","Peptide Chain Initiation, Translational","Ribosomes","RNA Caps","RNA, Viral","Protein Biosynthesis","Hepacivirus","Molecular Chaperones","Foot-and-Mouth Disease Virus","Hela Cells"],"keywords":["Internal ribosome entry site","Picornavirus","Foot-and-mouth disease virus","Translation (biology)","Ribosome","Biology","Virology","Protein biosynthesis","Eukaryotic translation","RNA","Cell biology","Virus","Messenger RNA","Molecular biology","Genetics","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-22T22:21:49.041558Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}