{"doi":"10.1016/j.fct.2021.112518","title":"A comprehensive study of the genotoxic and anti-genotoxic effects of homocysteine in HUVECs and mouse bone marrow cells","abstract":null,"journal":"Food and Chemical Toxicology","year":2021,"id":608176,"datarank":0.26876392038420827,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.0,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":329282,"name":"Yanmei Qi","orcid":"0009-0002-3222-6883","position":1,"is_corresponding":false},{"id":1202610,"name":"Jianfei Li","orcid":"0009-0007-4664-1187","position":2,"is_corresponding":false},{"id":1561852,"name":"Houhong Fan","orcid":null,"position":3,"is_corresponding":false},{"id":1561853,"name":"Limei Yang","orcid":null,"position":4,"is_corresponding":false},{"id":376371,"name":"Xue Wu","orcid":"0000-0002-0511-9436","position":5,"is_corresponding":false},{"id":1561856,"name":"Juan Ni","orcid":null,"position":6,"is_corresponding":false},{"id":406775,"name":"Han Wang","orcid":"0009-0004-6776-5013","position":7,"is_corresponding":false},{"id":337596,"name":"Xu Wang","orcid":"0000-0001-8428-9339","position":8,"is_corresponding":false},{"id":1561847,"name":"Xihan Guo","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A comprehensive study of the genotoxic and anti-genotoxic effects of homocysteine in HUVECs and mouse bone marrow cells","abstract":"Elevated Homocysteine (Hcy) is associated with increased risk of vascular disease, but whether it induces genotoxicity to vascular endothelial cells remains unknown. Here, we conducted a comprehensive study of the genotoxicity, and unexpected anti-genotoxicity, of Hcy by cytokinesis-blocked micronucleus assay in HUVECs and erythrocyte micronucleus test in mouse bone marrow cells. Our experiments led to several important findings. First, while supraphysiological Hcy (SP-Hcy) exhibited remarkable genotoxicity, physiologically-relevant Hcy (PR-Hcy) reduced the basal genotoxicity. Second, among the metabolites of Hcy, cysteine phenocopied the anti-genotoxicity of PR-Hcy and, methionine, S-adenosylhomocysteine and H<sub>2</sub>S phenocopied the genotoxicity of SP-Hcy. Third, the genotoxicity of SP-Hcy was mitigated by vitamin B<sub>6</sub>, Fe<sup>2+</sup> and Cu<sup>2+</sup>, but was exacerbated by N-acetylcysteine. Fourth, under pre-, co- or post-treatment protocol, both SP-Hcy and PR-Hcy attenuated the genotoxicity of cisplatin, mitomycin-C, nocodazole or deoxycholate. Finally, 100 and 250 mg/kg Hcy ameliorated cisplatin-induced genotoxicity in bone marrow cells of CF-1 and Kunming mice. Our results suggest that genotoxicity may be one mechanism through which Hcy confers an increased risk for vascular disease, but more importantly, they challenge the long-standing paradigm that Hcy is always harmful to human health. Our study calls for a more systematic effort in understanding the molecular mechanisms underlying the anti-genotoxicity of Hcy.","is_dataset_classified":null,"base_score":1.791759469228055,"endowment":1.791759469228055,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34418477","pmcid":null,"openalex_id":"https://openalex.org/W3194145227","authors":[],"funders":[],"total_grants":0,"fwci":0.7728,"citation_percentile":0.71198165,"influential_citations":0,"citation_trend":[{"year":2022,"count":2},{"year":2023,"count":1},{"year":2024,"count":2}],"oa_status":"closed","license":"https://doi.org/10.15223/policy-004","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0278691521005512?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0278691521005512?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.fct.2021.112518","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/34418477","host_type":"repository"}],"fields_of_study":["Folate and B Vitamins Research","Acute Lymphoblastic Leukemia research","Sulfur Compounds in Biology"],"mesh_terms":["Animals","Bone Marrow Cells","Copper","Dose-Response Relationship, Drug","Gene Expression Regulation","Homocysteine","Humans","Iron","Male","Mutagenicity Tests","Tetrahydrofolates","Vitamin B 6","Mice","Human Umbilical Vein Endothelial Cells"],"keywords":["Genotoxicity","Micronucleus test","Micronucleus","Pharmacology","Homocysteine","Bone marrow","Chemistry","Biology","Biochemistry","Immunology","Toxicity","micronuclei","Genotoxic Stress","Homocysteine Metabolism","Cytokinesis-blocked Micronucleus Assay","Mouse Bone Marrow Micronucleus Test"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T07:37:19.994556Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}