{"doi":"10.1016/j.exphem.2022.03.002","title":"CRISPR–Cas9 gene editing induced complex on-target outcomes in human cells","abstract":null,"journal":"Experimental Hematology","year":2022,"id":589373,"datarank":0.9962467700984357,"base_score":3.6109179126442243,"endowment":3.6109179126442243,"self_citation_contribution":0.5416376868966337,"citation_network_contribution":0.454609083201802,"self_endowment_contribution":0.5416376868966337,"citer_contribution":0.454609083201802,"corpus_percentile":null,"corpus_rank":null,"citation_count":36,"citer_count":30,"citers_with_citation_signal":24,"citers_with_endowment":24,"datacite_reuse_total":4,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1507949,"name":"Xiao-Bing Zhang","orcid":null,"position":1,"is_corresponding":false},{"id":641707,"name":"Wei Wen","orcid":"0000-0002-7911-9068","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"CRISPR–Cas9 gene editing induced complex on-target outcomes in human cells","abstract":"CRISPR-Cas9 is a powerful tool for editing the genome and holds great promise for gene therapy applications. Initial concerns of gene engineering focus on off-target effects. However, in addition to short indel mutations (often <50 bp), an increasing number of studies have revealed complex on-target results after double-strand break repair by CRISPR-Cas9, such as large deletions, gene rearrangement, and loss of heterozygosity. These unintended mutations are potential safety concerns in clinical gene editing. Here, in this review, we summarize the significant findings of CRISPR-Cas9-induced on-target deleterious outcomes and discuss putative ways to achieve safe gene therapy.","is_dataset_classified":null,"base_score":3.6109179126442243,"endowment":3.6109179126442243,"datacite_reuse_total":4,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"35304271","pmcid":null,"openalex_id":"https://openalex.org/W4220766053","authors":[],"funders":[{"funder_name":"CAMS Innovation Fund for Medical Sciences","grant_id":"","title":null},{"funder_name":"Special Funds for the Basic Research and Development Program in the Central Non-profit Research Institutes of China","grant_id":"","title":null}],"total_grants":2,"fwci":2.6638,"citation_percentile":0.91319594,"influential_citations":0,"citation_trend":[{"year":2022,"count":1},{"year":2023,"count":4},{"year":2024,"count":14},{"year":2025,"count":15},{"year":2026,"count":2}],"oa_status":"closed","license":"http://creativecommons.org/licenses/by-nc-nd/4.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0301472X22001242?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0301472X22001242?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.exphem.2022.03.002","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/35304271","host_type":"repository"}],"fields_of_study":["CRISPR and Genetic Engineering","Innovation and Socioeconomic Development","Genetics, Aging, and Longevity in Model Organisms","CRISPR-Cas Systems","DNA Repair","Gene Editing","Genetic Therapy","Genome","Humans"],"mesh_terms":["Gene Editing","DNA Repair","Humans","Genetic Therapy","Genome","CRISPR-Cas Systems"],"keywords":["CRISPR","Genome editing","Cas9","Computational biology","Biology","Gene","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[{"doi":"10.6084/m9.figshare.25239409.v1","title":"Additional file 1 of Decoding the complexity of on-target integration: characterizing DNA insertions at the CRISPR-Cas9 targeted locus using nanopore sequencing","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.25239409","title":"Additional file 1 of Decoding the complexity of on-target integration: characterizing DNA insertions at the CRISPR-Cas9 targeted locus using nanopore sequencing","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.25239456.v1","title":"Additional file 2 of Decoding the complexity of on-target integration: characterizing DNA insertions at the CRISPR-Cas9 targeted locus using nanopore sequencing","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.25239456","title":"Additional file 2 of Decoding the complexity of on-target integration: characterizing DNA insertions at the CRISPR-Cas9 targeted locus using nanopore sequencing","publisher":"figshare","resource_type":"JournalArticle"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-23T17:16:25.803583Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}