{"doi":"10.1016/j.exphem.2007.05.017","title":"Reversible cell surface expression of CD38 on CD34-positive human hematopoietic repopulating cells","abstract":null,"journal":"Experimental Hematology","year":2007,"id":672178,"datarank":0.586803450814222,"base_score":3.912023005428146,"endowment":3.912023005428146,"self_citation_contribution":0.586803450814222,"citation_network_contribution":0.0,"self_endowment_contribution":0.586803450814222,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":49,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":27379,"name":"Olga I. Gan","orcid":null,"position":1,"is_corresponding":false},{"id":1756173,"name":"Monica Doedens","orcid":null,"position":2,"is_corresponding":false},{"id":242536,"name":"John E. Dick","orcid":"0000-0002-9527-8317","position":3,"is_corresponding":false},{"id":1756172,"name":"Joby L. McKenzie","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Reversible cell surface expression of CD38 on CD34-positive human hematopoietic repopulating cells","abstract":"<h4>Objective</h4>Although increased expression of CD38 on the surface of human CD34(+) cells is associated with differentiation, we reported recently that both lineage-negative (Lin(-)) CD34(+)CD38(-) and Lin(-)CD34(+)CD38(lo) fractions of cord blood contain primitive severe combined immunodeficient (SCID)-repopulating cells (SRC). Thus, it is important to determine if a hierarchical relationship exists between the SRC from these two populations or if CD38 is reversibly expressed.<h4>Materials and methods</h4>To determine if SRC from the CD34(+)CD38(-) and CD34(+)CD38(lo) cell fractions could generate SRC of the same and/or alternate CD38 expression, cells from primary nonobese diabetic/SCID mice transplanted with CD34(+)CD38(-) cells were resorted into both CD34(+)CD38(-) and CD34(+)CD38(lo) fractions and injected into separate secondary recipients, which were evaluated for human cell engraftment 7 to 10 weeks later. As primary mice transplanted with CD34(+)CD38(lo) cells also contained cells of both immunophenotype, these cells were also resorted and transplanted into separate secondary recipients. The cell-cycle status of various CD34(+) SRC fractions were evaluated using Hoechst 33342 and Pyronin Y staining in order to determine if CD38 expression was coordinated with divisional activation.<h4>Results</h4>Each cell fraction obtained from primary recipients was able to reconstitute secondary mice, indicating that CD38 expression reversibly oscillates between negative and low levels on CD34(+) repopulating cells. CD38 expression on repopulating cells correlated with a transition between the G(0) and G(1) phases of the cell cycle.<h4>Conclusion</h4>CD38 is reversibly expressed on CD34(+) SRC between negative and low levels and corresponds to a change in the cell-cycle state. These observations establish a foundation to uncover the molecular program of stem cell regulation and underscore the importance of functional assessments when isolating and characterizing human hematopoietic stem cells.","is_dataset_classified":null,"base_score":3.912023005428146,"endowment":3.912023005428146,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17656009","pmcid":null,"openalex_id":"https://openalex.org/W2147652293","authors":[],"funders":[],"total_grants":0,"fwci":2.4111,"citation_percentile":0.89199864,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":3},{"year":2014,"count":2},{"year":2015,"count":1},{"year":2016,"count":4},{"year":2017,"count":5},{"year":2018,"count":3},{"year":2019,"count":1},{"year":2020,"count":2},{"year":2022,"count":1},{"year":2023,"count":2},{"year":2024,"count":1},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"closed","license":"http://www.elsevier.com/open-access/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0301472X07003475?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0301472X07003475?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.exphem.2007.05.017","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17656009","host_type":"repository"}],"fields_of_study":["Hematopoietic Stem Cell Transplantation","Calcium signaling and nucleotide metabolism","Mesenchymal stem cell research","ADP-ribosyl Cyclase 1","Animals","Antigens, CD34","Cell Culture Techniques","Cell Cycle","Hematopoiesis","Hematopoietic Stem Cells","Humans","Mice","Mice, SCID","Receptors, Cell Surface","Transplantation, Heterologous"],"mesh_terms":["Animals","Cell Cycle","Hematopoiesis","Hematopoietic Stem Cells","Humans","Receptors, Cell Surface","Transplantation, Heterologous","Mice, SCID","Hematopoietic Stem Cell Transplantation","Cell Culture Techniques","Antigens, CD34","Mice","ADP-ribosyl Cyclase 1"],"keywords":["CD38","Haematopoiesis","CD34","Biology","Stem cell","Molecular biology","Cell","Cell biology","Immunology","Chemistry","Biochemistry"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T07:53:12.192207Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}