{"doi":"10.1016/j.exer.2016.09.005","title":"Role of transcription factor Tgif2 in photoreceptor differentiation in the mouse retina","abstract":null,"journal":"Experimental Eye Research","year":2016,"id":675170,"datarank":0.26876392038420827,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.0,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1764112,"name":"Asano Tsuhako","orcid":null,"position":1,"is_corresponding":false},{"id":1764113,"name":"Mio Kikuchi","orcid":null,"position":2,"is_corresponding":false},{"id":1631021,"name":"Nobuaki Yoshida","orcid":null,"position":3,"is_corresponding":false},{"id":1764114,"name":"Hideto Koso","orcid":null,"position":4,"is_corresponding":false},{"id":975924,"name":"Sumiko Watanabe","orcid":"0000-0003-4497-5750","position":5,"is_corresponding":false},{"id":1764111,"name":"Hiroshi Kuribayashi","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Role of transcription factor Tgif2 in photoreceptor differentiation in the mouse retina","abstract":"5'TG3'-interacting factors (TGIFs) function as transcriptional repressors. Defects in TGIFs cause severe abnormalities in the developing brain and face. We found that Tgif2 was highly expressed in the mouse retina at early stages of development and examined its role in retinal development. Knockdown of Tgif2 in retinal explants at E14 using shRNA (sh-Tgif2) resulted in a decreased number of rod photoreceptors, whereas the number of cone photoreceptors increased without perturbation of cell proliferation and apoptosis. Concomitantly, the expression levels of photoreceptor-related genes were decreased in sh-Tgif2-introduced retinal explants. To examine the in vivo effects of Tgif2 overexpression, we generated Tgif2 conditional knock-in mice (Tgif2cKI). Although retinal cell differentiation, based on the relative proportions of retinal subtypes in the mature retina, was not affected, we observed abnormal localization of cone photoreceptor cell nuclei in the outer nuclear layer of the Tgif2cKI retina. However, electrical retinography suggest that cones in Tgif2cKI were functionally equivalent to those of wild mice. Our study revealed that Tgif2 participates in photoreceptor cell differentiation in the early stages of retinal development and regulates proper subretinal localization of the cone photoreceptors.","is_dataset_classified":null,"base_score":1.791759469228055,"endowment":1.791759469228055,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27639517","pmcid":null,"openalex_id":"https://openalex.org/W2519841065","authors":[],"funders":[{"funder_name":"Ministry of Education, Culture, Sports, Science and Technology","grant_id":"15H04695","title":null},{"funder_name":"Ministry of Education, Culture, Sports, Science and Technology","grant_id":"25118708","title":null}],"total_grants":2,"fwci":0.481,"citation_percentile":0.69958427,"influential_citations":0,"citation_trend":[{"year":2018,"count":1},{"year":2019,"count":2},{"year":2022,"count":1},{"year":2025,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0014483516302640?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0014483516302640?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.exer.2016.09.005","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27639517","host_type":"repository"}],"fields_of_study":["Retinal Development and Disorders","Developmental Biology and Gene Regulation","Ocular Disorders and Treatments","Animals","Disease Models, Animal","Electroretinography","Gene Expression Regulation, Developmental","Gene Knockdown Techniques","Homeodomain Proteins","In Situ Hybridization","Mice","Mice, Transgenic","Photoreceptor Cells, Vertebrate","RNA","Real-Time Polymerase Chain Reaction","Repressor Proteins","Retina","Retinal Diseases","Tissue Culture Techniques"],"mesh_terms":["Animals","Disease Models, Animal","Electroretinography","Mice, Transgenic","Repressor Proteins","Retina","Retinal Diseases","RNA","In Situ Hybridization","Homeodomain Proteins","Gene Expression Regulation, Developmental","Photoreceptor Cells, Vertebrate","Tissue Culture Techniques","Mice","Gene Knockdown Techniques","Real-Time Polymerase Chain Reaction"],"keywords":["Retina","Retinal","Outer nuclear layer","Biology","Visual phototransduction","Photoreceptor cell","Electroretinography","Cell biology","Retinal regeneration","Transcription factor","Genetics","Neuroscience","Gene","Biochemistry","Differentiation","Photoreceptors","Transcription factors","cone"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T20:06:11.706016Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}