{"doi":"10.1016/j.envint.2025.109755","title":"The association between glyphosate use and mosaic loss of chromosome Y in buccal samples among male pesticide applicators in the Agricultural Health Study","abstract":"Glyphosate-based herbicides are the most widely applied pesticides worldwide and have been implicated in the development of certain hematologic malignancies; however, the underlying biological mechanisms are not well-understood. High lifetime use of glyphosate-based herbicides, hereafter referred to as glyphosate, was previously associated with mosaic loss of chromosome Y (mLOY), a biomarker of genomic instability potentially linked to cancer and immune dysregulation, in circulating blood of male farmers from a subcohort of the Agricultural Health Study (AHS). Here, we further investigated the association between glyphosate use and mLOY using buccal-derived DNA among 1,868 male pesticide applicators in an independent AHS study. We detected mLOY using genotyping array intensity data in the male-specific region of the Y chromosome. Information on cumulative lifetime use of glyphosate was derived from questionnaires. Logistic regression analyses were used to estimate odds ratios (ORs) and 95 % confidence intervals (CIs) for the associations of glyphosate use with overall mLOY and mLOY affecting a high cell fraction (≥ median), adjusted for potential confounders. Overall, mLOY was detected in buccal samples of 298 (16 %) pesticide applicators. High intensity-weighted lifetime days of glyphosate use was associated with overall mLOY (OR highest vs. lowest quartile of glyphosate use = 1.54; 95 % CI: 1.01, 2.37), although the exposure–response trend was not statistically significant ( P trend = 0.40). For mLOY affecting a high fraction of cells, positive associations were observed for both high lifetime days (OR highest vs. lowest quartile of glyphosate use = 1.76; 95 % CI: 1.04, 2.99; P trend = 0.06) and high intensity-weighted lifetime days (OR highest vs. lowest quartile of glyphosate use = 2.02; 95 % CI: 1.12, 3.64; P trend = 0.16). Together, these findings support previous evidence linking glyphosate and mLOY and help inform our understanding of the biological impact of glyphosate on human health and its potential role in carcinogenesis.","journal":"Environment International","year":2025,"id":532855,"datarank":0.20794415416798362,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.956,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":240086,"name":"Stella Koutros","orcid":"0000-0001-5294-5485","position":1,"is_corresponding":false},{"id":314379,"name":"Lauren M. Hurwitz","orcid":"0000-0001-8932-5028","position":2,"is_corresponding":false},{"id":382571,"name":"Weiyin Zhou","orcid":"0000-0002-0467-3064","position":3,"is_corresponding":false},{"id":104585,"name":"Gabriella Andreotti","orcid":"0000-0002-1096-3324","position":4,"is_corresponding":false},{"id":247401,"name":"Christine G. Parks","orcid":"0000-0002-5734-3456","position":5,"is_corresponding":false},{"id":104612,"name":"Dale P. Sandler","orcid":"0000-0002-6776-0018","position":6,"is_corresponding":false},{"id":96418,"name":"Nathaniel Rothman","orcid":"0000-0001-6924-0895","position":7,"is_corresponding":false},{"id":227671,"name":"Mitchell J. Machiela","orcid":"0000-0001-6538-9705","position":8,"is_corresponding":false},{"id":104588,"name":"Laura E. Beane Freeman","orcid":"0000-0003-1294-4124","position":9,"is_corresponding":false},{"id":241832,"name":"Jonathan N. Hofmann","orcid":"0000-0002-1043-5812","position":10,"is_corresponding":false},{"id":21999,"name":"Sonja I. Berndt","orcid":"0000-0001-5230-0652","position":11,"is_corresponding":false},{"id":939113,"name":"Vicky C. Chang","orcid":"0000-0002-2528-9387","position":0,"is_corresponding":true}],"reference_count":54,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:51:27.893975Z","pmid":"40914106","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}