{"doi":"10.1016/j.engmic.2023.100101","title":"CRISPR-based gene editing technology and its application in microbial engineering","abstract":null,"journal":"Engineering Microbiology","year":2023,"id":600529,"datarank":0.6862066467755076,"base_score":4.574710978503383,"endowment":4.574710978503383,"self_citation_contribution":0.6862066467755076,"citation_network_contribution":0.0,"self_endowment_contribution":0.6862066467755076,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":96,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":755032,"name":"Yingjun Li","orcid":"0000-0002-4835-8074","position":1,"is_corresponding":false},{"id":1539636,"name":"Junwei Wei","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"CRISPR-based gene editing technology and its application in microbial engineering","abstract":"Gene editing technology involves the modification of a specific target gene to obtain a new function or phenotype. Recent advances in clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-mediated technologies have provided an efficient tool for genetic engineering of cells and organisms. Here, we review the three emerging gene editing tools (ZFNs, TALENs, and CRISPR-Cas) and briefly introduce the principle, classification, and mechanisms of the CRISPR-Cas systems. Strategies for gene editing based on endogenous and exogenous CRISPR-Cas systems, as well as the novel base editor (BE), prime editor (PE), and CRISPR-associated transposase (CAST) technologies, are described in detail. In addition, we summarize recent developments in the application of CRISPR-based gene editing tools for industrial microorganism and probiotics modifications. Finally, the potential challenges and future perspectives of CRISPR-based gene editing tools are discussed.","is_dataset_classified":null,"base_score":4.574710978503383,"endowment":4.574710978503383,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"39628916","pmcid":"PMC11610974","openalex_id":"https://openalex.org/W4381325555","authors":[],"funders":[{"funder_name":"National Natural Science Foundation of China","grant_id":"32170096","title":null},{"funder_name":"Fundamental Research Funds for the Central Universities","grant_id":"2662022SKPY001","title":null}],"total_grants":2,"fwci":8.9647,"citation_percentile":0.98827952,"influential_citations":0,"citation_trend":[{"year":2023,"count":9},{"year":2024,"count":13},{"year":2025,"count":47},{"year":2026,"count":27}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://doi.org/10.1016/j.engmic.2023.100101","host_type":"journal"},{"url":"https://doi.org/10.1016/j.engmic.2023.100101","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2667370323000334?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2667370323000334?httpAccept=text/plain","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/39628916","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11610974","host_type":"repository"},{"url":"https://doaj.org/article/0e4f17e2c3d742a080c9c2af1b74342b","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11610974/pdf/main.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC11610974","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC11610974?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["CRISPR and Genetic Engineering","Bacterial Genetics and Biotechnology","Animal Genetics and Reproduction"],"mesh_terms":[],"keywords":["CRISPR","Genome editing","Transcription activator-like effector nuclease","Computational biology","Zinc finger nuclease","Cas9","Biology","Gene","Palindrome","Computer science","Genetics","Probiotics","Gene Editing","Crispr-cas System","Industrial Microorganisms"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Industry, innovation and infrastructure"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T13:24:35.171222Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}