{"doi":"10.1016/j.ekir.2020.01.018","title":"Proteomic Analysis of Complement Proteins in Membranous Nephropathy","abstract":"INTRODUCTION: Membranous nephropathy (MN) is the most common cause of nephrotic syndrome in Caucasian adults. Phospholipase A2 receptor (PLA2R)- and exostosin 1 (EXT1)/exostosin 2 (EXT2)-associated MN represent the most common primary and secondary forms of MN. The complement profile using a proteomics approach has not been studied in these 2 common forms of MN. METHODS: = 21), and 11 control cases (time 0 transplant biopsies). RESULTS: MS/MS identified high total spectral counts for PLA2R and EXT1/EXT2 in corresponding cases of PLA2R- and EXT1/EXT2-positive MN. Both PLA2R- and EXT1/EXT2-associated MN had high spectral counts of complement proteins C3, C4, C5, C6, C7, C8, and C9. Complement protein C1 was present in low spectral counts in EXT1/EXT2-associated MN. Regulators of complement activation that were detected in MN included higher spectral counts of FH, FHR-1, FHR-5, clusterin, vitronectin and lower spectral counts of FHR-3, FHR-4, and CD59. Low spectral counts of FB and properdin, key components of the alternative pathway, also were detected. IgG4 and IgG1 were the most abundant IgG subclasses in PLA2R- and EXT1/EXT2-associated MN. Lower spectral counts for C3, C4, and C5 were detected in control cases when compared with MN. CONCLUSION: Significant complement activation is present in MN as evidenced by large spectral counts of complement proteins from C3- and C4-based pathways, including regulatory proteins of complement pathways. These data suggest that anticomplement drugs may be effective in treatment for MN.","journal":"Kidney International Reports","year":2020,"id":57247,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":79,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.5873,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":298642,"name":"Benjamin J. Madden","orcid":"0000-0001-9167-8535","position":1,"is_corresponding":false},{"id":298643,"name":"M. Cristine Charlesworth","orcid":"0000-0001-7481-817X","position":2,"is_corresponding":false},{"id":298644,"name":"Rishi Sharma","orcid":"0000-0003-3757-0368","position":3,"is_corresponding":false},{"id":298645,"name":"Amit Sethi","orcid":"0000-0003-2813-2016","position":4,"is_corresponding":false},{"id":298646,"name":"Hanna Dêbiec","orcid":"0000-0002-8946-8245","position":5,"is_corresponding":false},{"id":244120,"name":"Daniel Cattran","orcid":null,"position":6,"is_corresponding":false},{"id":242666,"name":"Fernando C. Fervenza","orcid":"0000-0002-9952-209X","position":7,"is_corresponding":false},{"id":291758,"name":"Richard J. Smith","orcid":"0000-0003-1201-6731","position":8,"is_corresponding":false},{"id":109005,"name":"Pierre Ronco","orcid":"0000-0002-0280-6900","position":9,"is_corresponding":false},{"id":298647,"name":"Sanjeev Sethi","orcid":"0000-0002-4536-7709","position":10,"is_corresponding":false},{"id":298641,"name":"Aishwarya Ravindran","orcid":"0000-0003-4503-440X","position":0,"is_corresponding":true}],"reference_count":24,"raw_metadata":null,"created_at":"2026-07-18T21:06:31.136481Z","pmid":"32405583","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}