{"doi":"10.1016/j.ejphar.2025.178331","title":"Neuroprotection by post-stroke administration of the slow-releasing hydrogen sulfide (H2S) donor AP39: Long-Term functional, MRI, and molecular evidence in a rodent stroke model","abstract":null,"journal":"European Journal of Pharmacology","year":2025,"id":616358,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1589012,"name":"Krzyżanowska Weronika","orcid":null,"position":1,"is_corresponding":false},{"id":1589013,"name":"Skórkowska Alicja","orcid":"0000-0002-1167-7610","position":2,"is_corresponding":false},{"id":1589014,"name":"Jurczyk Jakub","orcid":null,"position":3,"is_corresponding":false},{"id":979065,"name":"Katarzyna Przejczowska‐Pomierny","orcid":"0000-0001-9833-5885","position":4,"is_corresponding":false},{"id":1589015,"name":"Szafarz Małgorzata","orcid":null,"position":5,"is_corresponding":false},{"id":1589016,"name":"Marcinkowska Monika","orcid":null,"position":6,"is_corresponding":false},{"id":1589017,"name":"Guzda Zuzanna","orcid":null,"position":7,"is_corresponding":false},{"id":1589018,"name":"Rachwał Patrycja","orcid":null,"position":8,"is_corresponding":false},{"id":1589019,"name":"Kim Eugene","orcid":null,"position":9,"is_corresponding":false},{"id":1589020,"name":"Mesquita Michel","orcid":"0000-0002-5594-5605","position":10,"is_corresponding":false},{"id":1589021,"name":"Torregrossa Roberta","orcid":null,"position":11,"is_corresponding":false},{"id":1589022,"name":"Whiteman Matthew","orcid":null,"position":12,"is_corresponding":false},{"id":1589023,"name":"Pomierny-Chamioło Lucyna","orcid":"0000-0002-8308-8030","position":13,"is_corresponding":false},{"id":557597,"name":"Joanna Pera","orcid":"0000-0001-6142-2969","position":14,"is_corresponding":false},{"id":1589024,"name":"Budziszewska Bogusława","orcid":null,"position":15,"is_corresponding":false},{"id":769088,"name":"Bartosz Pomierny","orcid":"0000-0002-5352-5324","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Neuroprotection by post-stroke administration of the slow-releasing hydrogen sulfide (H2S) donor AP39: Long-Term functional, MRI, and molecular evidence in a rodent stroke model","abstract":"Hydrogen sulfide (H<sub>2</sub>S) is an endogenous gaseous signalling molecule with potential cytoprotective properties in neurological disorders. AP39, a mitochondria-targeted H<sub>2</sub>S donor, has previously demonstrated neuroprotection in stroke models. Here, we investigated the therapeutic efficacy of AP39 administered after stroke in a rat model of transient middle cerebral artery occlusion (MCAO). AP39 (100 nmol/kg) was delivered intravenously 10 min after reperfusion. Animals underwent longitudinal assessments at 24 h and 7 days post-stroke, including neurological deficit assessment, grid-walking test, MRI (T2-weighted, diffusion tensor imaging [DTI], perfusion-weighted imaging [PWI]), and molecular analyses (qRT-PCR, Western blot, Luminex). AP39 treatment significantly reduced infarct volume at both 24 h and 7 d. A reduction in infarct core was observed at 24 h, while penumbra volume was decreased at 7 d. Treated animals showed improved neurological and motor function, preserved blood-brain barrier integrity, and attenuated microstructural damage based on fractional anisotropy (FA) and mean diffusivity (MD) metrics. Post-stroke administration of AP39 improved neurological and MRI outcomes and was accompanied by reduced neuroinflammation, including a significant decrease in the number of activated microglia. AP39 also downregulated apoptotic markers while upregulating neurotrophic factors at 24 h. Collectively, our findings provide substantial evidence supporting the therapeutic potential of AP39 when administered post-ischemia, thereby highlighting its therapeutic potential in mitigating the detrimental consequences of ischemic stroke.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"41241336","pmcid":null,"openalex_id":"https://openalex.org/W4416439074","authors":[],"funders":[{"funder_name":"National Science Centre Poland","grant_id":"2016/21/D/NZ4/03302","title":null},{"funder_name":"National Science Centre Poland","grant_id":"2023/50/E/NZ7/00586","title":null}],"total_grants":2,"fwci":0.0,"citation_percentile":0.2553096,"influential_citations":0,"citation_trend":[],"oa_status":"green","license":"cc-by-nc-nd","oa_locations":[{"url":"https://ruj.uj.edu.pl/handle/item/565648","host_type":"repository"},{"url":"https://ruj.uj.edu.pl/handle/item/565648","host_type":"repository"},{"url":"https://api.elsevier.com/content/article/PII:S0014299925010854?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0014299925010854?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.ejphar.2025.178331","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/41241336","host_type":"repository"}],"fields_of_study":["Sulfur Compounds in Biology","Hydrogen's biological and therapeutic effects","Redox biology and oxidative stress","Animals","Male","Hydrogen Sulfide","Neuroprotective Agents","Rats","Disease Models, Animal","Infarction, Middle Cerebral Artery","Magnetic Resonance Imaging","Brain","Rats, Sprague-Dawley","Stroke","Organophosphorus Compounds","Thiones"],"mesh_terms":["Animals","Brain","Disease Models, Animal","Hydrogen Sulfide","Magnetic Resonance Imaging","Male","Organophosphorus Compounds","Thiones","Rats, Sprague-Dawley","Neuroprotective Agents","Infarction, Middle Cerebral Artery","Stroke","Rats"],"keywords":["Neuroprotection","Penumbra","Stroke (engine)","Fractional anisotropy","Diffusion MRI","Ischemia","Oxidative stress","Rodent model","Brain ischemia","MRI","Hydrogen sulfide","MCAO","neuroinflammation","Penumbra Volume"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T22:40:43.867298Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}