{"doi":"10.1016/j.ebr.2023.100642","title":"Aerobic exercise alters DNA hydroxymethylation levels in an experimental rodent model of temporal lobe epilepsy","abstract":"The therapeutic potential of aerobic exercise in mitigating seizures and cognitive issues in temporal lobe epilepsy (TLE) is recognized, yet the underlying mechanisms are not well understood. Using a rodent TLE model induced by Kainic acid (KA), we investigated the impact of a single bout of exercise (i.e., acute) or 4 weeks of aerobic exercise (i.e., chronic). Blood was processed for epilepsy-associated serum markers, and DNA methylation (DNAme), and hippocampal area CA3 was assessed for gene expression levels for DNAme-associated enzymes. While acute aerobic exercise did not alter serum Brain-Derived Neurotrophic Factor (BDNF) or Interleukin-6 (IL-6), chronic exercise resulted in an exercise-specific decrease in serum BDNF and an increase in serum IL-6 levels in epileptic rats. Additionally, whole blood DNAme levels, specifically 5-hydroxymethylcytosine (5-hmC), decreased in epileptic animals following chronic exercise. Hippocampal CA3 5-hmC levels and ten-eleven translocation protein (TET1) expression mirrored these changes. Furthermore, immunohistochemistry analysis revealed that most 5-hmC changes in response to chronic exercise were neuron-specific within area CA3 of the hippocampus. Together, these findings suggest that DNAme mechanisms in the rodent model of TLE are responsive to chronic aerobic exercise, with emphasis on neuronal 5-hmC DNAme in the epileptic hippocampus.","journal":"Epilepsy & Behavior Reports","year":2023,"id":350677,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9618,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":719538,"name":"Rudhab Bahabry","orcid":"0000-0002-3748-2766","position":1,"is_corresponding":false},{"id":926084,"name":"Anna Maria Schreiber","orcid":"0000-0002-2404-4003","position":2,"is_corresponding":false},{"id":1095491,"name":"J Homola","orcid":null,"position":3,"is_corresponding":false},{"id":1095492,"name":"Tram Ngyuen","orcid":null,"position":4,"is_corresponding":false},{"id":1095493,"name":"Fernando Meijia","orcid":null,"position":5,"is_corresponding":false},{"id":427724,"name":"Jane B. Allendorfer","orcid":"0000-0001-6888-9000","position":6,"is_corresponding":false},{"id":563164,"name":"Farah D. Lubin","orcid":"0000-0003-0234-6956","position":7,"is_corresponding":false},{"id":1095072,"name":"Silvienne Sint Jago","orcid":"0000-0001-7997-8105","position":0,"is_corresponding":true}],"reference_count":99,"raw_metadata":null,"created_at":"2026-07-19T01:12:37.409066Z","pmid":"38323091","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}