{"doi":"10.1016/j.diagmicrobio.2025.117017","title":"Pathogen epidemiological study of hospitalized children with acute respiratory infections based on targeted next-generation sequencing","abstract":null,"journal":"Diagnostic Microbiology and Infectious Disease","year":2025,"id":608347,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1562379,"name":"Yinju Jian","orcid":null,"position":1,"is_corresponding":false},{"id":1562380,"name":"Guying Guo","orcid":null,"position":2,"is_corresponding":false},{"id":1562381,"name":"Zufu Cheng","orcid":null,"position":3,"is_corresponding":false},{"id":1562382,"name":"Shewen Jiang","orcid":null,"position":4,"is_corresponding":false},{"id":1562383,"name":"Shaorong Wu","orcid":null,"position":5,"is_corresponding":false},{"id":1562384,"name":"Shumin Chen","orcid":null,"position":6,"is_corresponding":false},{"id":214298,"name":"Wenming Zhang","orcid":"0000-0002-9575-7713","position":7,"is_corresponding":false},{"id":796051,"name":"Jun Tang","orcid":"0000-0002-5746-1778","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Pathogen epidemiological study of hospitalized children with acute respiratory infections based on targeted next-generation sequencing","abstract":"<h4>Background</h4>Acute respiratory infections (ARIs) remain a leading cause of pediatric hospitalization, yet their etiological complexity and age-specific epidemiological patterns are poorly characterized.<h4>Methods</h4>This study utilized targeted next-generation sequencing (tNGS) to investigate the prevalence, seasonality, and age-dependent dynamics of 108 respiratory pathogens in 1,468 hospitalized children with ARIs.<h4>Results</h4>Totally, 64.7 % were tested positive for viruses and 77.2 % were positive for bacteria. The top three viruses were human rhinovirus (18.5 %), human respiratory syncytial virus B (7.7 %) and human adenovirus B (7.0 %), and the predominant bacteria were Haemophilus influenzae (35.6 %), Mycoplasmoides pneumoniae (27.9 %) and Streptococcus pneumoniae (21.6 %). The tNGS additionally identified pathogens such as enterovirus (4.1 %), parechovirus A (1.2 %), dengue virus (1.0 %), and Bordetella pertussis (2.0 %). Viral and bacterial detection rates exhibited different age-dependent patterns, and thereby substantially influenced coinfection dynamics. Seasonal trends also revealed significant variability, with certain pathogens peaking during winter-spring months and others demonstrating sustained prevalence across the year.<h4>Conclusion</h4>These findings provided a comprehensive framework for understanding ARIs pathogenesis, and emphasized the need for age-tailored diagnostics, seasonal surveillance, and antimicrobial stewardship to optimize clinical management in pediatric populations.","is_dataset_classified":null,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"40706102","pmcid":null,"openalex_id":"https://openalex.org/W4412654941","authors":[],"funders":[],"total_grants":0,"fwci":0.8521,"citation_percentile":0.75442571,"influential_citations":0,"citation_trend":[{"year":2026,"count":1}],"oa_status":"closed","license":"https://doi.org/10.15223/policy-004","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0732889325003402?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0732889325003402?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.diagmicrobio.2025.117017","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/40706102","host_type":"repository"}],"fields_of_study":["Respiratory viral infections research","Pneumonia and Respiratory Infections","Influenza Virus Research Studies"],"mesh_terms":["Acute Disease","Adolescent","Bacteria","Bacterial Infections","Child","Child, Hospitalized","Child, Preschool","Female","Hospitalization","Humans","Infant","Infant, Newborn","Male","Respiratory Tract Infections","Seasons","Virus Diseases","Viruses","Prevalence","High-Throughput Nucleotide Sequencing","Coinfection"],"keywords":["Epidemiology","Pathogen","Pathogenic organism","Medicine","Intensive care medicine","Biology","Virology","Microbiology","Immunology","Internal medicine","Children","Acute respiratory infections","Targeted Next-generation Sequencing"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T12:05:32.255607Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}