{"doi":"10.1016/j.diagmicrobio.2017.08.001","title":"Evaluation of quantitative FTD- Pneumocystis jirovecii kit for Pneumocystis infection diagnosis","abstract":null,"journal":"Diagnostic Microbiology and Infectious Disease","year":2017,"id":666135,"datarank":0.44166584687496613,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"self_citation_contribution":0.44166584687496613,"citation_network_contribution":0.0,"self_endowment_contribution":0.44166584687496613,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1400089,"name":"Solène Le Gal","orcid":"0000-0003-0747-9571","position":1,"is_corresponding":false},{"id":1739582,"name":"Patricia Zunic","orcid":null,"position":2,"is_corresponding":false},{"id":1739584,"name":"Patrice Poubeau","orcid":null,"position":3,"is_corresponding":false},{"id":1739586,"name":"Emmanuel Antok","orcid":null,"position":4,"is_corresponding":false},{"id":1739588,"name":"Julien Jaubert","orcid":null,"position":5,"is_corresponding":false},{"id":1400088,"name":"Gilles Nevez","orcid":"0000-0001-5888-6017","position":6,"is_corresponding":false},{"id":1739589,"name":"Sandrine Picot","orcid":null,"position":7,"is_corresponding":false},{"id":1739579,"name":"Gautier Hoarau","orcid":"0000-0002-4667-0697","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Evaluation of quantitative FTD- Pneumocystis jirovecii kit for Pneumocystis infection diagnosis","abstract":"We evaluated the Fast track Diagnostics (FTD) Pneumocystis PCR kit, targeting the mitochondrial large subunit ribosomal RNA gene (mtLSU rRNA) of Pneumocystis jirovecii (P. jirovecii). A hundred and thirty-three patients were prospectively enrolled. Respiratory specimens were examined using both microscopy and the PCR assay. Twenty-six patients led to P. jirovecii detection. Fourteen patients presented with Pneumocystis pneumonia (PCP) whereas 12 patients were considered to be colonized. The median copy numbers in bronchoalveolar lavage fluid were significantly different in the PCP and colonization groups (1.35×10<sup>8</sup>/ml vs. 1.45×10<sup>5</sup>/ml, P < 0.0001). Lower and upper cut-off values of 3.9×10<sup>5</sup> copies/ml and 3.2×10<sup>6</sup> copies/ml allowed differentiating PCP and colonization. The FTD P. jirovecii assay was secondarily compared to an in-house reference PCR assay targeting the mtLSUrRNA gene. A concordance rate of 97.5% was observed (Cohen's kappa coefficient κ=0.935). The FTD Pneumocystis PCR kit showed good performance and represents an alternative method to diagnose P. jirovecii infections.","is_dataset_classified":null,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"28851493","pmcid":null,"openalex_id":"https://openalex.org/W2743868684","authors":[],"funders":[],"total_grants":0,"fwci":1.1458,"citation_percentile":0.7827529,"influential_citations":0,"citation_trend":[{"year":2018,"count":1},{"year":2019,"count":3},{"year":2020,"count":3},{"year":2021,"count":3},{"year":2022,"count":2},{"year":2023,"count":4},{"year":2025,"count":2}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://doi.org/10.1016/j.diagmicrobio.2017.08.001","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/28851493","host_type":"repository"},{"url":"https://univ-angers.hal.science/hal-02407803","host_type":"repository"}],"fields_of_study":["Pneumocystis jirovecii pneumonia detection and treatment","HIV/AIDS drug development and treatment","Tuberculosis Research and Epidemiology"],"mesh_terms":["Bronchoalveolar Lavage Fluid","Female","Humans","Male","Middle Aged","Pneumonia, Pneumocystis","Prospective Studies","RNA, Fungal","RNA, Ribosomal","Sensitivity and Specificity","Pneumocystis carinii","Real-Time Polymerase Chain Reaction"],"keywords":["Pneumocystis jirovecii","Pneumocystis pneumonia","Pneumocystis carinii","Bronchoalveolar lavage","Concordance","Biology","Ribosomal RNA","Virology","Medicine","Gene","Internal medicine","Lung","Human immunodeficiency virus (HIV)","Genetics","PCR","Colonization"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-13T13:26:52.627002Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}