{"doi":"10.1016/j.devcel.2024.12.025","title":"A single-nuclei transcriptome census of the Arabidopsis maturing root identifies that MYB67 controls phellem cell maturation","abstract":"The periderm provides a protective barrier in many seed plant species. The development of the suberized phellem, which forms the outermost layer of this important tissue, has become a trait of interest for enhancing both plant resilience to stresses and plant-mediated CO 2 sequestration in soils. Despite its importance, very few genes driving phellem development are known. Employing single-nuclei sequencing, we have generated an expression census capturing the complete developmental progression of Arabidopsis root phellem cells, from their progenitor cell type, the pericycle, through to their maturation. With this, we identify a whole suite of genes underlying this process, including MYB67 , which we show has a role in phellem cell maturation. Our expression census and functional discoveries represent a resource, expanding our comprehension of secondary growth in plants. These data can be used to fuel discoveries and engineering efforts relevant to plant resilience and climate change. • scRNA-seq produced a comprehensive expression census of the maturing Arabidopsis root • Distinct phellem developmental stages are captured across multiple nuclei populations • Dynamic shifts in gene expression and biological processes drive phellem development • MYB67 , identified in the maturing root census, controls phellem cell maturation Using small nuclear RNA sequencing (snRNA-seq), Miller et al. produced a gene expression census of the maturing Arabidopsis root and, by exploring the development of the suberizing phellem, identify that MYB67 controls phellem cell maturation. This census may serve as resource for engineering future plants with improved stress resilience and carbon capture capacity.","journal":"Developmental Cell","year":2025,"id":516007,"datarank":0.5080142420624696,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"self_citation_contribution":0.3596842909197557,"citation_network_contribution":0.1483299511427139,"self_endowment_contribution":0.3596842909197557,"citer_contribution":0.1483299511427139,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":10,"citers_with_citation_signal":7,"citers_with_endowment":7,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9507,"is_data_producer":true,"deposit_databanks":{"GEO":["GSE261441","GSE261431","GSE261437"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1381366,"name":"Sean Jarrell-Hurtado","orcid":null,"position":1,"is_corresponding":false},{"id":1381367,"name":"Manisha V. Haag","orcid":null,"position":2,"is_corresponding":false},{"id":1380878,"name":"Yiwei Ye","orcid":"0000-0002-1227-1241","position":3,"is_corresponding":false},{"id":1381368,"name":"Mathew Simenc","orcid":null,"position":4,"is_corresponding":false},{"id":1381369,"name":"Paloma Alvarez-Maldonado","orcid":null,"position":5,"is_corresponding":false},{"id":1381370,"name":"Sara Behnami","orcid":null,"position":6,"is_corresponding":false},{"id":314452,"name":"Ling Zhang","orcid":"0000-0001-8412-2010","position":7,"is_corresponding":false},{"id":680680,"name":"Joseph Swift","orcid":"0000-0001-9559-1699","position":8,"is_corresponding":false},{"id":605584,"name":"Ashot Papikian","orcid":"0000-0002-2801-6341","position":9,"is_corresponding":false},{"id":550353,"name":"Jingting Yu","orcid":"0009-0005-1182-3680","position":10,"is_corresponding":false},{"id":1326034,"name":"Kelly Colt","orcid":"0009-0004-9349-4325","position":11,"is_corresponding":false},{"id":3799,"name":"Joseph R. Ecker","orcid":"0000-0001-5799-5895","position":12,"is_corresponding":false},{"id":28822,"name":"Todd P. Michael","orcid":"0000-0001-6272-2875","position":13,"is_corresponding":false},{"id":283069,"name":"Julie A. Law","orcid":"0000-0001-7472-7753","position":14,"is_corresponding":false},{"id":249596,"name":"Wolfgang Busch","orcid":"0000-0003-2042-7290","position":15,"is_corresponding":false},{"id":776255,"name":"Charlotte Miller","orcid":"0000-0003-2228-1619","position":0,"is_corresponding":true}],"reference_count":75,"raw_metadata":null,"created_at":"2026-07-19T02:48:44.647022Z","pmid":"39793584","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}