{"doi":"10.1016/j.devcel.2024.01.005","title":"PUF partner interactions at a conserved interface shape the RNA-binding landscape and cell fate in Caenorhabditis elegans","abstract":"Protein-RNA regulatory networks underpin much of biology. C. elegans FBF-2, a PUF-RNA-binding protein, binds over 1,000 RNAs to govern stem cells and differentiation. FBF-2 interacts with multiple protein partners via a key tyrosine, Y479. Here, we investigate the in vivo significance of partnerships using a Y479A mutant. Occupancy of the Y479A mutant protein increases or decreases at specific sites across the transcriptome, varying with RNAs. Germline development also changes in a specific fashion: Y479A abolishes one FBF-2 function-the sperm-to-oocyte cell fate switch. Y479A's effects on the regulation of one mRNA, gld-1, are critical to this fate change, though other network changes are also important. FBF-2 switches from repression to activation of gld-1 RNA, likely by distinct FBF-2 partnerships. The role of RNA-binding protein partnerships in governing RNA regulatory networks will likely extend broadly, as such partnerships pervade RNA controls in virtually all metazoan tissues and species.","journal":"Developmental Cell","year":2024,"id":464690,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9631,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":438899,"name":"Sarah L. Crittenden","orcid":null,"position":1,"is_corresponding":false},{"id":451100,"name":"Fan Chen","orcid":"0000-0003-3873-7094","position":2,"is_corresponding":false},{"id":1295622,"name":"MaryGrace Linsley","orcid":"0009-0000-7698-7647","position":3,"is_corresponding":false},{"id":1295939,"name":"Jennifer Woodworth","orcid":null,"position":4,"is_corresponding":false},{"id":1295940,"name":"Peggy Kroll-Conner","orcid":null,"position":5,"is_corresponding":false},{"id":1096735,"name":"Ahlan Sabah Ferdous","orcid":"0000-0002-3610-304X","position":6,"is_corresponding":false},{"id":43259,"name":"Sündüz Keleş","orcid":"0000-0001-9048-0922","position":7,"is_corresponding":false},{"id":255523,"name":"Marvin Wickens","orcid":"0000-0002-0593-5740","position":8,"is_corresponding":false},{"id":438022,"name":"Judith Kimble","orcid":"0000-0001-5622-2073","position":9,"is_corresponding":false},{"id":1145286,"name":"Brian H. Carrick","orcid":"0000-0003-0139-3582","position":0,"is_corresponding":true}],"reference_count":130,"raw_metadata":null,"created_at":"2026-07-19T02:04:42.082401Z","pmid":"38290520","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}