{"doi":"10.1016/j.devcel.2019.01.020","title":"Assessment and Maintenance of Unigametic Germline Inheritance for C. elegans","abstract":null,"journal":"Developmental Cell","year":2019,"id":635113,"datarank":0.519860385419959,"base_score":3.4657359027997265,"endowment":3.4657359027997265,"self_citation_contribution":0.519860385419959,"citation_network_contribution":0.0,"self_endowment_contribution":0.519860385419959,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":31,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1647569,"name":"Andrew Z. Fire","orcid":null,"position":1,"is_corresponding":false},{"id":1647572,"name":"Christian Frøkjær-Jensen","orcid":null,"position":2,"is_corresponding":false},{"id":1175848,"name":"Karen L. Artiles","orcid":"0000-0002-1674-064X","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Assessment and Maintenance of Unigametic Germline Inheritance for C. elegans","abstract":"The recent work of Besseling and Bringmann (2016) identified a molecular intervention for C. elegans in which premature segregation of maternal and paternal chromosomes in the fertilized oocyte can produce viable animals exhibiting a non-Mendelian inheritance pattern. Overexpression in embryos of a single protein regulating chromosome segregation (GPR-1) provides a germline derived clonally from a single parental gamete. We present a collection of strains and cytological assays to consistently generate and track non-Mendelian inheritance. These tools allow reproducible and high-frequency (>80%) production of non-Mendelian inheritance, the facile and simultaneous homozygosis for all nuclear chromosomes in a single generation, the precise exchange of nuclear and mitochondrial genomes between strains, and the assessments of non-canonical mitosis events. We show the utility of these strains by demonstrating a rapid assessment of cell lineage requirements (AB versus P1) for a set of genes (lin-2, lin-3, lin-12, and lin-31) with roles in C. elegans vulval development.","is_dataset_classified":null,"base_score":3.4657359027997265,"endowment":3.4657359027997265,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30799227","pmcid":"PMC6435406","openalex_id":"https://openalex.org/W2916948412","authors":[],"funders":[{"funder_name":"NIGMS","grant_id":"R01-GM37706","title":null},{"funder_name":"NIGMS","grant_id":"GM130366","title":null},{"funder_name":"NIH","grant_id":"P40 OD010440","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R35 GM130366","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM037706","title":null}],"total_grants":5,"fwci":1.2091,"citation_percentile":0.74296963,"influential_citations":0,"citation_trend":[{"year":2017,"count":1},{"year":2019,"count":3},{"year":2020,"count":4},{"year":2021,"count":2},{"year":2022,"count":6},{"year":2023,"count":6},{"year":2024,"count":2},{"year":2025,"count":4},{"year":2026,"count":3}],"oa_status":"bronze","license":"cc-by-nc-nd","oa_locations":[{"url":"http://www.cell.com/article/S1534580719300486/pdf","host_type":"journal"},{"url":"http://www.cell.com/article/S1534580719300486/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1534580719300486?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1534580719300486?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.devcel.2019.01.020","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/30799227","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6435406","host_type":"repository"},{"url":"http://hdl.handle.net/10754/631308","host_type":"repository"}],"fields_of_study":["Genetics, Aging, and Longevity in Model Organisms","Mitochondrial Function and Pathology","Photosynthetic Processes and Mechanisms","Animals","Biomarkers","Caenorhabditis elegans","Caenorhabditis elegans Proteins","Chromosomes","Crosses, Genetic","Female","Fluorescence","Germ Cells","Inheritance Patterns","Male","Microtubules","Mosaicism","Mutation","Pharynx","Phenotype","Vulva","Zygote"],"mesh_terms":["Animals","Chromosomes","Crosses, Genetic","Female","Fluorescence","Germ Cells","Male","Microtubules","Mosaicism","Mutation","Pharynx","Phenotype","Vulva","Zygote","Biomarkers","Caenorhabditis elegans","Caenorhabditis elegans Proteins","Inheritance Patterns"],"keywords":["Biology","Genetics","Germline","Mendelian inheritance","Non-Mendelian inheritance","Gamete","Mitosis","Meiosis","Inheritance (genetic algorithm)","Caenorhabditis elegans","Genome","Chromosome","Gene","Lineage (genetic)","Mitochondrial DNA","Sperm","Genetic engineering","Inheritance","Synthetic Biology","Mosaic","C. elegans","Non-mendelian"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"bioproject"},{"name":"doi"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T14:39:08.969169Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}