{"doi":"10.1016/j.cub.2014.04.048","title":"Transcriptional Regulation by Pho23 Modulates the Frequency of Autophagosome Formation","abstract":null,"journal":"Current Biology","year":2014,"id":620969,"datarank":0.692268077526189,"base_score":4.61512051684126,"endowment":4.61512051684126,"self_citation_contribution":0.692268077526189,"citation_network_contribution":0.0,"self_endowment_contribution":0.692268077526189,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":100,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":878410,"name":"Ding He","orcid":"0000-0001-9620-6115","position":1,"is_corresponding":false},{"id":1603219,"name":"Steven K. Backues","orcid":null,"position":2,"is_corresponding":false},{"id":1603220,"name":"Mallory A. Freeberg","orcid":null,"position":3,"is_corresponding":false},{"id":366621,"name":"Xu Liu","orcid":"0000-0003-2252-7148","position":4,"is_corresponding":false},{"id":1603223,"name":"John K. Kim","orcid":null,"position":5,"is_corresponding":false},{"id":1603224,"name":"Daniel J. Klionsky","orcid":null,"position":6,"is_corresponding":false},{"id":567208,"name":"Meiyan Jin","orcid":"0000-0002-7506-1989","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Transcriptional Regulation by Pho23 Modulates the Frequency of Autophagosome Formation","abstract":"<h4>Background</h4>Autophagy as a conserved lysosomal/vacuolar degradation and recycling pathway is important in normal development and physiology, and defects in this process are linked to many kinds of disease. Because too much or too little autophagy can be detrimental, the process must be tightly regulated both temporally and in magnitude. Two parameters that affect this regulation are the size and the number of autophagosomes; however, although we know that the amount of Atg8 affects the size of autophagosomes, the mechanism for regulating their number has not been elucidated. The transcriptional induction and repression of the autophagy-related (ATG) genes is one crucial aspect of autophagy regulation, but the transcriptional regulators that modulate autophagy are not well characterized.<h4>Results</h4>We detected increased expression levels of ATG genes, and elevated autophagy activity, in cells lacking the transcriptional regulator Pho23. Using transmission electron microscopy, we found that PHO23 null mutant cells contain significantly more autophagosomes than the wild-type. By RNA sequencing transcriptome profiling, we identified ATG9 as one of the key targets of Pho23, and our studies with strains expressing modulated levels of Atg9 show that the amount of this protein directly correlates with the frequency of autophagosome formation and the level of autophagy activity.<h4>Conclusions</h4>Our results identified Pho23 as a master transcriptional repressor for autophagy that regulates the frequency of autophagosome formation through its negative regulation of ATG9.","is_dataset_classified":null,"base_score":4.61512051684126,"endowment":4.61512051684126,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24881874","pmcid":"PMC4169046","openalex_id":"https://openalex.org/W2111423166","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM088565","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM053396","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM088565","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM053396","title":null},{"funder_name":"National Science Foundation","grant_id":"0903629","title":"IGERT: Open Data: Graduate Training for Data Sharing and Reuse in E-Science"},{"funder_name":"National Institutes of Health","grant_id":"9R01GM053396-05","title":"ALTERNATIVE VACUOLAR TARGETING MECHANISMS IN YEAST"}],"total_grants":6,"fwci":5.2857,"citation_percentile":0.96169561,"influential_citations":0,"citation_trend":[{"year":2014,"count":3},{"year":2015,"count":6},{"year":2016,"count":9},{"year":2017,"count":8},{"year":2018,"count":8},{"year":2019,"count":15},{"year":2020,"count":11},{"year":2021,"count":11},{"year":2022,"count":10},{"year":2023,"count":9},{"year":2024,"count":7},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":"Elsevier Non-Commercial","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0960982214004862?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0960982214004862?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.cub.2014.04.048","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/24881874","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4169046","host_type":"repository"},{"url":"http://www.cell.com/article/S0960982214004862/pdf","host_type":""},{"url":"http://dx.doi.org/10.1016/j.cub.2014.04.048","host_type":""},{"url":"https://dx.doi.org/10.1016/j.cub.2014.04.048","host_type":""}],"fields_of_study":["Autophagy in Disease and Therapy","Cellular transport and secretion","Lysosomal Storage Disorders Research","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Autophagy-Related Proteins","Autophagy","Membrane Proteins","Nuclear Proteins","Phagosomes","Saccharomyces cerevisiae","Gene Expression Regulation, Fungal","Gene Expression Profiling","Saccharomyces cerevisiae Proteins","Microscopy, Electron, Transmission","Real-Time Polymerase Chain Reaction"],"keywords":["Autophagy","Biology","Cell biology","ATG8","Autophagosome","BAG3","Transcriptome","Transcriptional regulation","Regulator","Regulation of gene expression","Psychological repression","Repressor","Gene","Gene expression","Genetics","Apoptosis","Saccharomyces cerevisiae Proteins","Agricultural and Biological Sciences(all)","Biochemistry, Genetics and Molecular Biology(all)","Gene Expression Profiling","Autophagy-Related Proteins","Membrane Proteins","Nuclear Proteins","Saccharomyces cerevisiae","Real-Time Polymerase Chain Reaction","Microscopy, Electron, Transmission","Gene Expression Regulation, Fungal","Phagosomes"],"sdg_mappings":[{"sdg_number":12,"sdg_label":"12. 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