{"doi":"10.1016/j.cub.2008.10.044","title":"The GTPase-Activating Protein GRAF1 Regulates the CLIC/GEEC Endocytic Pathway","abstract":null,"journal":"Current Biology","year":2008,"id":589057,"datarank":7.664453898704177,"base_score":5.54907608489522,"endowment":5.54907608489522,"self_citation_contribution":0.8323614127342831,"citation_network_contribution":6.832092485969894,"self_endowment_contribution":0.8323614127342831,"citer_contribution":6.832092485969894,"corpus_percentile":null,"corpus_rank":null,"citation_count":256,"citer_count":200,"citers_with_citation_signal":179,"citers_with_endowment":179,"datacite_reuse_total":4,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1014861,"name":"Gary J. Doherty","orcid":"0000-0002-8158-8111","position":1,"is_corresponding":false},{"id":1507065,"name":"Mark T. Howes","orcid":null,"position":2,"is_corresponding":false},{"id":879982,"name":"Katia Cortese","orcid":"0000-0001-9218-8933","position":3,"is_corresponding":false},{"id":1507066,"name":"Yvonne Vallis","orcid":null,"position":4,"is_corresponding":false},{"id":151227,"name":"Robert G. Parton","orcid":"0000-0002-7494-5248","position":5,"is_corresponding":false},{"id":1507067,"name":"Harvey T. McMahon","orcid":null,"position":6,"is_corresponding":false},{"id":1480833,"name":"Richard Lundmark","orcid":"0000-0001-9104-724X","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The GTPase-Activating Protein GRAF1 Regulates the CLIC/GEEC Endocytic Pathway","abstract":"Clathrin-independent endocytosis is an umbrella term for a variety of endocytic pathways that internalize numerous cargoes independently of the canonical coat protein Clathrin [1, 2]. Electron-microscopy studies have defined the pleiomorphic CLathrin-Independent Carriers (CLICs) and GPI-Enriched Endocytic Compartments (GEECs) as related major players in such uptake [3, 4]. This CLIC/GEEC pathway relies upon cellular signaling and activation through small G proteins, but mechanistic insight into the biogenesis of its tubular and tubulovesicular carriers is lacking. Here we show that the Rho-GAP-domain-containing protein GRAF1 marks, and is indispensable for, a major Clathrin-independent endocytic pathway. This pathway is characterized by its ability to internalize bacterial exotoxins, GPI-linked proteins, and extracellular fluid. We show that GRAF1 localizes to PtdIns(4,5)P2-enriched, tubular, and punctate lipid structures via N-terminal BAR and PH domains. These membrane carriers are relatively devoid of caveolin1 and flotillin1 but are associated with activity of the small G protein Cdc42. This study provides the first specific noncargo marker for CLIC/GEEC endocytic membranes and demonstrates how GRAF1 can coordinate small G protein signaling and membrane remodeling to facilitate internalization of CLIC/GEEC pathway cargoes.","is_dataset_classified":null,"base_score":5.54907608489522,"endowment":5.54907608489522,"datacite_reuse_total":4,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19036340","pmcid":"PMC2726289","openalex_id":"https://openalex.org/W2001674490","authors":[],"funders":[{"funder_name":"Medical Research Council","grant_id":"MC_U105178795","title":null}],"total_grants":1,"fwci":6.4639,"citation_percentile":0.97439346,"influential_citations":0,"citation_trend":[{"year":2012,"count":14},{"year":2013,"count":14},{"year":2014,"count":22},{"year":2015,"count":13},{"year":2016,"count":8},{"year":2017,"count":16},{"year":2018,"count":9},{"year":2019,"count":14},{"year":2020,"count":24},{"year":2021,"count":22},{"year":2022,"count":10},{"year":2023,"count":9},{"year":2024,"count":9},{"year":2025,"count":12},{"year":2026,"count":7}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"http://www.cell.com/article/S0960982208014139/pdf","host_type":"journal"},{"url":"http://www.cell.com/article/S0960982208014139/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0960982208014139?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0960982208014139?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1016/j.cub.2008.10.044","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/19036340","host_type":"repository"},{"url":"https://hdl.handle.net/11567/228691","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2726289","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC2726289","host_type":"Europe_PMC"}],"fields_of_study":["Cellular transport and secretion","Glycosylation and Glycoproteins Research","Cell Adhesion Molecules Research","Animals","Endocytosis","GTPase-Activating Proteins","HeLa Cells","Humans","Lipid Metabolism","Mice","NIH 3T3 Cells","Protein Structure, Tertiary","Rats","Signal Transduction"],"mesh_terms":["Animals","Endocytosis","HeLa Cells","Humans","Signal Transduction","Protein Structure, Tertiary","GTPase-Activating Proteins","NIH 3T3 Cells","Lipid Metabolism","Mice","Rats","Hela Cells"],"keywords":["Biology","Endocytic cycle","GTPase","Cell biology","GTPase-activating protein","Signal transduction","Genetics","Endocytosis","Cell","G protein"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[{"doi":"10.6084/m9.figshare.13192083.v1","title":"Additional file 2 of Clathrin- and dynamin-dependent endocytosis limits canonical NF-κB signaling triggered by lymphotoxin β receptor","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.13192083","title":"Additional file 2 of Clathrin- and dynamin-dependent endocytosis limits canonical NF-κB signaling triggered by lymphotoxin β receptor","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.13192086.v1","title":"Additional file 3 of Clathrin- and dynamin-dependent endocytosis limits canonical NF-κB signaling triggered by lymphotoxin β receptor","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.13192086","title":"Additional file 3 of Clathrin- and dynamin-dependent endocytosis limits canonical NF-κB signaling triggered by lymphotoxin β receptor","publisher":"figshare","resource_type":"JournalArticle"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-23T12:22:09.837869Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}