{"doi":"10.1016/j.crsus.2023.100009","title":"Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat production","abstract":"Cultivated meat (also known as cultured meat) is a promising technology that faces substantial cost barriers, which are currently driven by the price of media components. Particularly, growth factors such as fibroblast growth factor 2 (FGF2) drive the cost of serum-free media for relevant cells, including muscle satellite cells. Here, we engineered immortalized bovine satellite cells (iBSCs) for inducible expression of FGF2 and/or mutant RasG12V to overcome media growth factor requirements through autocrine signaling. Engineered cells were able to proliferate over multiple passages in FGF2-free medium, thereby eliminating the need for this costly component. Additionally, cells maintained their myogenicity, albeit with reduced myotube formation. Ultimately, this offers a proof of principle for lower-cost cultured meat production through cell line engineering.","journal":"Cell Reports Sustainability","year":2024,"id":422804,"datarank":0.4887144807032224,"base_score":3.258096538021482,"endowment":3.258096538021482,"self_citation_contribution":0.4887144807032224,"citation_network_contribution":0.0,"self_endowment_contribution":0.4887144807032224,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":25,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9489,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1160368,"name":"Xiaoli Zhang","orcid":"0009-0004-4269-4685","position":1,"is_corresponding":false},{"id":660669,"name":"Sophia M. Letcher","orcid":"0000-0003-4280-5520","position":2,"is_corresponding":false},{"id":842756,"name":"Miriam L. Rittenberg","orcid":null,"position":3,"is_corresponding":false},{"id":842757,"name":"Michelle Shub","orcid":null,"position":4,"is_corresponding":false},{"id":1160970,"name":"Kristin M. Chai","orcid":null,"position":5,"is_corresponding":false},{"id":1160369,"name":"Maya Kaul","orcid":"0000-0002-9933-5956","position":6,"is_corresponding":false},{"id":225373,"name":"David L. Kaplan","orcid":"0000-0002-9245-7774","position":7,"is_corresponding":false},{"id":256654,"name":"Andrew J. Stout","orcid":"0000-0001-9470-1032","position":0,"is_corresponding":true}],"reference_count":43,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:57:51.642883Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}