{"doi":"10.1016/j.crmeth.2025.101113","title":"Volumetric imaging and computation to explore contractile function in zebrafish hearts","abstract":"Novel insights into cardiac contractile dysfunction at the cellular level could deepen understanding of arrhythmia and heart injury, which are leading causes of morbidity and mortality worldwide. We present a comprehensive experimental and computational framework combining light-field microscopy and single-cell tracking to investigate real-time volumetric data in live zebrafish hearts, which share structural and electrical similarities to the human heart. Our system acquires 200 vol/s with lateral resolution of up to 5.02 ± 0.54 μm and axial resolution of 9.02 ± 1.11 μm across the whole depth using an expectation-maximization-smoothed deconvolution algorithm. We apply a deep-learning approach to quantify cell displacement and velocity in blood flow and myocardial motion and to perform real-time volumetric tracking from end-systole to end-diastole within a virtual reality environment. This capability delivers high-speed and high-resolution imaging of cardiac contractility at single-cell resolution over multiple cycles, supporting in-depth investigation of intercellular interactions in health and disease.","journal":"Cell Reports Methods","year":2025,"id":545516,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.6581,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1436786,"name":"Riya P. Patel","orcid":null,"position":1,"is_corresponding":false},{"id":1081824,"name":"Milad Almasian","orcid":"0009-0001-4159-8825","position":2,"is_corresponding":false},{"id":961458,"name":"Xinyuan Zhang","orcid":"0009-0008-1914-3024","position":3,"is_corresponding":false},{"id":704609,"name":"Jonathan R. Brewer","orcid":"0000-0002-3444-1715","position":4,"is_corresponding":false},{"id":907160,"name":"Sohail S. Hassan","orcid":"0000-0001-5340-2503","position":5,"is_corresponding":false},{"id":1436787,"name":"Jichen Chai","orcid":null,"position":6,"is_corresponding":false},{"id":89806,"name":"Juhyun Lee","orcid":"0000-0002-9609-4376","position":7,"is_corresponding":false},{"id":254797,"name":"Baowei Fei","orcid":"0000-0002-9123-9484","position":8,"is_corresponding":false},{"id":765438,"name":"Jie Yuan","orcid":"0000-0002-7789-6744","position":9,"is_corresponding":false},{"id":383530,"name":"Kelli J. Carroll","orcid":"0000-0001-6120-8321","position":10,"is_corresponding":false},{"id":385040,"name":"Yichen Ding","orcid":"0000-0002-6242-3506","position":11,"is_corresponding":false},{"id":1106906,"name":"Alireza Saberigarakani","orcid":"0009-0008-7748-0424","position":0,"is_corresponding":true}],"reference_count":66,"raw_metadata":null,"created_at":"2026-07-19T02:53:23.001995Z","pmid":"40706593","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}