{"doi":"10.1016/j.crmeth.2025.101109","title":"High-throughput measurement of Drosophila feeding behavior","abstract":null,"journal":"Cell Reports Methods","year":2025,"id":631477,"datarank":0.16479184330021646,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.0,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1636490,"name":"Shuo Song","orcid":null,"position":1,"is_corresponding":false},{"id":1636491,"name":"Jing-Hao Tang","orcid":null,"position":2,"is_corresponding":false},{"id":1636492,"name":"Hong-Shan Liu","orcid":null,"position":3,"is_corresponding":false},{"id":1636493,"name":"Man Song","orcid":null,"position":4,"is_corresponding":false},{"id":52149,"name":"Zhen‐Xia Chen","orcid":"0000-0003-0474-902X","position":5,"is_corresponding":false},{"id":1636489,"name":"Huai-Zheng Zheng","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"High-throughput measurement of Drosophila feeding behavior","abstract":"Accurate measurement of Drosophila feeding is vital for metabolic and aging studies, but current assays lack the throughput and sensitivity needed for large-scale screens. We introduce FlyPlate-BCA, a 96-well platform that combines automated single-fly tracking with BCA protein quantification for high-resolution, longitudinal intake measurements. FlyPlate-BCA detects a 0.1× nutrient dilution with 90% power using just four replicates, three times fewer than the capillary feeder (CAFE) assay. It uncovers sucrose-driven hyperphagia independent of osmotic stress and preserved lipid-satiety responses, while extended monitoring reveals a female-specific metabolic adaptation. By delivering individual-level data at scale, FlyPlate-BCA establishes a benchmark for nutritional genomics and appetite neurobiology and streamlines nutraceutical and genetic screening.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"40683246","pmcid":"PMC12461584","openalex_id":null,"authors":[],"funders":[{"funder_name":"Fundamental Research Funds for the Central Universities","grant_id":"2662023JC004","title":null},{"funder_name":"National Natural Science Foundation of China","grant_id":"32470711","title":null}],"total_grants":2,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":null,"license":"http://creativecommons.org/licenses/by-nc-nd/4.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S2667237525001456?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S2667237525001456?httpAccept=text/plain","host_type":"publisher"},{"url":"https://europepmc.org/articles/PMC12461584","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC12461584?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":[],"mesh_terms":["Animals","Drosophila","Drosophila melanogaster","Sucrose","Feeding Behavior","Female","Male","High-Throughput Screening Assays"],"keywords":["High-throughput screening","Cp: Metabolism","Sucrose Hyperphagia","Drosophila Feeding","Flyplate-bca","Sex-specific Intake"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-05T23:43:28.025365Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}